Article

CXCL12 enhances exogenous CD4+CD25+ T cell migration and prevents embryo loss in non-obese diabetic mice.

Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou, People's Republic of China.
Fertility and sterility (impact factor: 3.97). 05/2008; 91(6):2687-96. DOI:10.1016/j.fertnstert.2008.01.109 pp.2687-96
Source: PubMed

ABSTRACT To investigate the possible role of CXCL12 in the migration of regulatory T (Treg) cells.
Animal model-based study.
Academic.
Pregnant non-obese diabetic (NOD) mice were compared with non-immunodeficient mice.
In vivo and in vitro CXCL12 induction.
Flow cytometric analysis and Treg cell migratory assay.
A significantly high percentage of spontaneous embryo resorption was observed in both syngeneic and allogeneic pregnant NOD mice. The percentage of embryo loss in allogeneic pregnant NOD mice was significantly decreased by treatment with Treg cells and CXCL12 injection; however, no such effect was observed in syngeneic pregnant NOD mice. In addition, the migration of Treg cells induced by CXCL12 was confirmed by both in vitro and in vivo migratory assays. CXCR4, the specific receptor for CXCL12, was expressed more intensively on Treg cells than on non-Treg CD3(+) T cells, whereas CXCL12 was dominantly expressed in cytokeratin 7(+) trophoblast cells at an early stage of gestation, and its expression reduced gradually during pregnancy.
The higher level of embryo loss in allogeneic pregnant NOD mice may be due to the lack of Treg cells. CXCL12 can cause CXCR4(+) Treg cells to migrate into the pregnant uterus and establish a beneficial microenvironment for the fetus.

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Keywords

allogeneic pregnant NOD mice
 
Animal model-based study
 
fetus
 
Flow cytometric analysis
 
migrate
 
NOD
 
non-immunodeficient mice
 
possible role
 
Pregnant non-obese diabetic
 
pregnant uterus
 
regulatory T
 
specific receptor
 
spontaneous embryo resorption
 
syngeneic
 
syngeneic pregnant NOD mice
 
Treg cell migratory assay
 
Treg cells
 
Treg cells induced
 
vivo
 
vivo migratory assays
 

Yi Lin