Curcumin, a constituent of curry, suppresses IgE-mediated allergic response and mast cell activation at the level of Syk

Department of Immunology and physiology, College of Medicine, Konkuk University, Chungju, Korea.
The Journal of allergy and clinical immunology (Impact Factor: 11.48). 06/2008; 121(5):1225-31. DOI: 10.1016/j.jaci.2007.12.1160
Source: PubMed


Activation of mast cells through the high-affinity receptor for IgE (FcepsilonRI) underlies atopic allergic reactions. Curcumin can block this activation, but the mechanism and the effects of curcumin on IgE-mediated allergic reactions are unknown.
We sought to determine the antiallergic activity of curcumin in vivo and its mechanism of action in mast cells.
The antiallergic activity of curcumin was evaluated in mast cell cultures and the passive cutaneous anaphylaxis model. The effects of curcumin on mast cell signaling events were examined by using immunoblotting, immunoprecipitation, RT-PCR, and other molecular biologic approaches.
Curcumin inhibited antigen-mediated activation of mast cells and passive cutaneous anaphylaxis in mice. Suppression of degranulation and secretion of TNF-alpha and IL-4 was apparent at concentrations as low as 3 micromol/L curcumin in activated mast cells. Similar concentrations of curcumin suppressed Syk-dependent phosphorylations of the adaptor proteins linker of activated T cells and Grb2-associated binder 2, which are critical for mast cell activation. Although curcumin did not inhibit the phosphorylation of Syk itself, it directly inhibited Syk kinase activity in vitro. Further downstream, activating phosphorylations of Akt and the mitogen-activated protein kinases p38, p44/42 (extracellular signal-regulated kinase 1/2), and c-Jun N-terminal kinase, which are critical for the production of inflammatory cytokines, were also inhibited.
Curcumin inhibits Syk kinase-dependent signaling events in mast cells and might thus contribute to its antiallergic activity. Therefore curcumin might be useful for the treatment of mast cell-related immediate and delayed allergic diseases.

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    • "Anaphylaxis is an IgE-mediated hypersensitivity reaction caused by the release of various inflammatory mediators due to binding of specific IgE to FcεRI on the surfaces of mast cells or basophils (Siraganian, 2003). Furthermore, it has been reported that curcumin suppresses IgE or compound 48/80 induced passive cutaneous anaphylaxis (Suzuki et al., 2005; Lee et al., 2008; Choi et al., 2010). However, no report has been issued on the effect of curcumin on PSA in mice. "
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    • "Curcumin inhibited FcεRI signaling to exert an anti-allergic effect by directly inhibiting Syk kinase activity in antigen-stimulated mast cells (10). In addition, curcumin suppressed degranulation and secretion of TNF-α and IL-4 in activated mast cells in a passive cutaneous anaphylaxis mice model (10). Taken together, these reports suggest that curcumin exerts its beneficial effect on inflammatory skin diseases by multiple distinct mechanisms. "
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    • "Curcumin is a yellow polyphenol compound isolated from the rhizomes of Curcuma longa, a plant that grows in India, China, and Southeast Asia.9,10 In the past few years, studies have shown that curcumin has potential therapeutic value in a variety of chronic diseases, including asthma.11 "
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