Synthesis of 2'-O-beta-d-Ribofuranosylnucleosides.
ABSTRACT A simple and efficient method for the preparation of 2-O-beta-D-ribofuranosylnucleosides, minor tRNA components, is described in this unit. The method consists of condensation of a small excess of 1-O-acetyl-2,3,5-tri-O-benzoyl-beta-D-ribofuranose activated with tin tetrachloride with N-protected 3,5-O-tetra-isopropyldisiloxane-1,3-diyl-ribonucleosides in 1,2-dichloroethane. Subsequent deprotection produces 2-O-beta-D-ribofuranosylnucleosides in an overall yield of 46% to 72%.
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ABSTRACT: The main structural features of disaccharide nucleosides, an important group of natural compounds, are reviewed. The preparation and properties of modified oligonucleotides constructed on their basis and the incorporation of reactive groups are summarized. Several examples are given for the use of the compounds to investigate the enzymes of nucleic acid metabolism.Molecular Biology 43(2):301-312. · 0.64 Impact Factor