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Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging.

Evan W Miller, Qiwen He, Christopher J Chang

Department of Chemistry, University of California, Berkeley, California 94720, USA.

Journal Article: Nature Protocol 02/2008; 3(5):777-83. DOI: 10.1038/nprot.2008.43

Abstract

Leadfluor-1 (LF1) is a small-molecule fluorescent sensor for detecting lead in biological and environmental samples, including live cells. This dye uses a xanthenone fluorescent scaffold coupled to a dicarboxylate pseudocrown ether receptor to achieve selective detection of Pb(2+) in the presence of biologically relevant metal ions, including divalent calcium, magnesium and zinc. LF1 fluorescence increases by up to 18-fold on binding Pb(2+). In this protocol, we describe the synthesis and application of LF1 to imaging lead accumulation within live cells. The preparation of LF1 is anticipated to take 14-21 d, and the imaging assays can be performed in 1-2 d with cultured cells.

Source: PubMed

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Keywords

biologically relevant metal ions
 
cultured cells
 
detecting lead
 
dicarboxylate pseudocrown ether receptor
 
divalent calcium
 
Leadfluor-1
 
LF1 fluorescence increases
 
selective detection
 
xanthenone fluorescent scaffold