Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow

Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT, USA.
Methods in Molecular Biology (Impact Factor: 1.29). 02/2007; 412:239-56. DOI: 10.1007/978-1-59745-467-4_16
Source: PubMed


Neutrophil migration from blood into tissues is required for effective innate immune responses against infection. Adhesion of the neutrophil in blood to the vascular endothelium and eventual migration through the vessel wall and accumulation at the site of infection involves different classes of adhesion molecules. In vivo intravital microscopy studies show that different adhesion molecules mediate binding events under shear forces associated with blood flow vs binding events that take place under static conditions. To fully analyze the function of these adhesion molecules in vitro, assays must reflect the hemodynamic forces associated with blood flow. We outline two approaches used to study neutrophil adhesion under conditions that mimic blood flow.

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Available from: Bruce Walcheck, May 27, 2014
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    • "However, these static assays lack the in vivo complex microcirculation environment, in which the presence of physiological fluid flow and geometric features can determine spatiotemporal changes in hemodynamic conditions and factors impacting adhesion (Fahim, 2003). Therefore, in recent years in vitro flow chambers characterized by a simple geometry and defined flow conditions have been used to study the adhesive interactions between particles/cells and adhesion molecules of the endothelium (Decuzzi et al., 2007; Haun and Hammer, 2008; Jutila et al., 2007; Sang et al., 2007; Sperandio et al., 2006). With the advancement of MEMS-based microfluidic systems during the last few years, micro-scale flow chambers have been developed to accurately reproduce the in vivo conditions (e.g. "
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