Inactivation of Avian Influenza Virus Using Common Detergents and Chemicals

Department of Bioresources Engineering, University of Delaware, 264 Townsend Hall, Newark, DE 19716, USA.
Avian Diseases (Impact Factor: 1.11). 04/2008; 52(1):118-23. DOI: 10.1637/8055-070907-Reg
Source: PubMed

ABSTRACT Six disinfectant chemicals were tested individually for effectiveness against low pathogenic avian influenza virus (LPAIV) A/H7N2/Chick/MinhMa/04. The tested agents included acetic acid (C2H4O2), citric acid (C6H8O7), calcium hypochlorite (Ca(ClO)2), sodium hypochlorite (NaOCl), a powdered laundry detergent with peroxygen (bleach), and a commercially available iodine/acid disinfectant. Four of the six chemicals, including acetic acid (5%), citric acid (1% and 3%), calcium hypochlorite (750 ppm), and sodium hypochlorite (750 ppm) effectively inactivated LPAIV on hard and nonporous surfaces. The conventional laundry detergent was tested at multiple concentrations and found to be suitable for inactivating LPAIV on hard and nonporous surfaces at 6 g/L. Only citric acid and commercially available iodine/acid disinfectant were found to be effective at inactivating LPAIV on both porous and nonporous surfaces.

1 Follower
  • Source
    • "Chlorine-based surface disinfectants are routinely used and are effective against many viruses, including influenza A virus (Lombardi et al. 2008). 750 ppm concentration of sodium hypochlorite is required for a 3 log PFU inactivation of influenza A virus within 10 min (Lombardi et al. 2008). For a variety of settings where chlorine demand cannot be controlled or high chlorine concentrations cannot be used, for this reason alternative sanitizers are needed. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Influenza A virus poses a major public health concern and is associated with annual epidemics and occasional pandemics. Influenza A H3N2 viruses, which are an important cause of human influenza, can infect birds and mammals. Contaminated undercooked poultry products including eggs with avian influenza virus constitute a possible risk of transmission to humans. In this study, a novel levulinic acid plus sodium dodecyl sulfate (SDS) sanitizer was evaluated for eggshell decontamination. Influenza A H3N2 virus-inoculated chicken eggshells were treated with a 5 % levulinic acid plus 2 % SDS, 2 % levulinic acid plus 1 % SDS, and 0.5 % levulinic acid plus 0.5 % SDS liquid solution for 1 min. Log reductions of viable viruses were observed by plaque assay. The 5 % levulinic acid plus 2 % SDS sanitizer provided the greatest level of influenza A H3N2 virus inactivation (2.23 log PFU), and differences in virus inactivation were observed for the various levulinic acid plus SDS concentrations tested (P ≤ 0.05). To the best of our knowledge, this is the first study demonstrating influenza A H3N2 virus inactivation on eggshells using a novel levulinic acid plus SDS sanitizer. The sanitizer may be useful for reducing egg contamination and preventing the spread of avian influenza virus to humans.
    Food and Environmental Virology 10/2013; 5(4). DOI:10.1007/s12560-013-9129-x · 1.98 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To control the spread of avian flu (influenza) and other viruses of concern among commercial flocks, it is essential that proper disinfection procedures be developed along with methods for assessing their performance. Such methods must be rapid and inexpensive. Coliphages were used as indicators to demonstrate the efficacy of quaternary ammonium compounds and chlorine bleach for the inactivation of viruses in chicken cages. The concentration of indigenous coliphages in chicken litter was found to be 104–107 per gram and from 0 to 8,500 per 100cm2 of floor surface. To assess the effectiveness of the disinfectants, floor samples were collected pre and post disinfection. These results indicated that chlorine bleach was more effective than quaternary ammonium compounds in reducing the amount of indigenous coliphages. To obtain better quantitative data, MS-2 coliphage was sprayed onto cage floors, left overnight to dry, and then the surfaces disinfected. Similar results were obtained with both indigenous coliphages and MS-2. There appears to be no significant difference in coliphage reduction by increasing the contact time from 10 to 30min. To ensure at least a 99.9% reduction of virus at least 236ml of household bleach per 3.78l should be used.
    Food and Environmental Virology 12/2009; 1(3):155-160. DOI:10.1007/s12560-009-9022-9 · 1.98 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Ceramic powder prepared by sintering of chicken feces, when mixed with avian influenza viruses or an avian adenovirus, inactivated these organisms to below detection levels. When the ceramic powder was mixed with double-distilled water, the pH of the water rose to 10 but the aqueous phase did not show any antivirus activity. After 10 washings with water or five washings with 1M Tris-HCl (pH 8.0), the ceramic powder still retained antivirus activity. Antivirus activity was not affected by the presence of organic material (33% fetal calf serum). When chicks were fed food containing 5% ceramic powder, there was no difference in body weight between normal feeding and the ceramic-mixture feeding. The mode of action of the ceramic powder remains unknown, but it possibly works by adsorbing the virus. These results show that the ceramic powder has antiviral activities and is a potentially useful tool against avian influenza on poultry farms.
    Avian Diseases 04/2009; 53(1):34-8. DOI:10.1637/8599.1 · 1.11 Impact Factor
Show more