Article

Chicken B lymphoma DT40 cells as a useful tool for in vitro analysis of pathogenic infectious bursal disease virus.

Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
Journal of Veterinary Medical Science (impact factor: 0.85). 05/2008; 70(4):407-10.
Source: PubMed

ABSTRACT Susceptibility of DT40 cells to pathogenic field strains of infectious bursal disease virus (IBDV) including very virulent and classical virulent strains were studied. After the first and second passage of the virus in DT40 cells, IBDV-specific antigen was readily detected in DT40 cells inoculated with the pathogenic field strain infected bursal homogenates. Nucleotide sequence analysis in the VP2 hypervariable domain, which is critical for the virulence of IBDV, revealed no common amino acid substitutions among the pathogenic IBDVs in accordance with the propagation in DT40 cells. These results indicate that DT40 cells are a useful tool for rapid isolation of pathogenic field strains and successive in vitro analysis of IBDV.

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Keywords

classical virulent strains
 
common amino acid substitutions
 
critical
 
DT40 cells
 
DT40 cells inoculated
 
IBDV
 
IBDV-specific antigen
 
infectious bursal disease virus
 
Nucleotide sequence analysis
 
pathogenic field strain
 
pathogenic field strains
 
pathogenic IBDVs
 
second passage
 
successive
 
useful tool
 
virulent
 
VP2 hypervariable domain