Construction of a binary vector for knockout and expression analysis of rice blast fungus genes.

Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
Bioscience Biotechnology and Biochemistry (Impact Factor: 1.27). 06/2008; 72(5):1380-3. DOI: 10.1271/bbb.70834
Source: PubMed

ABSTRACT We developed an efficient method to analyze gene function and expression of the rice blast fungus. We constructed a GATEWAY binary vector, which generates a gene-targeted disruptant carrying a green fluorescent protein gene under the native promoter of the target gene. Using this method, the knockout efficiency and expression patterns of two hypothetical genes were determined.

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