A Live Attenuated Severe Acute Respiratory Syndrome Coronavirus Is Immunogenic and Efficacious in Golden Syrian Hamsters

Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, MD 20892, USA.
Journal of Virology (Impact Factor: 4.44). 09/2008; 82(15):7721-4. DOI: 10.1128/JVI.00304-08
Source: PubMed

ABSTRACT The immunogenicity and protective efficacy of a live attenuated vaccine consisting of a recombinant severe acute respiratory syndrome (SARS) coronavirus lacking the E gene (rSARS-CoV-DeltaE) were studied using hamsters. Hamsters immunized with rSARS-CoV-DeltaE developed high serum-neutralizing antibody titers and were protected from replication of homologous (SARS-CoV Urbani) and heterologous (GD03) SARS-CoV in the upper and lower respiratory tract. rSARS-CoV-DeltaE-immunized hamsters remained active following wild-type virus challenge, while mock-immunized hamsters displayed decreased activity. Despite being attenuated in replication in the respiratory tract, rSARS-CoV-DeltaE is an immunogenic and efficacious vaccine in hamsters.

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Available from: AJ Roberts, Feb 18, 2015
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    • "Weight loss is difficult to assess in hamsters due to the storage of food in large cheek pouches; however, the use of a running wheel with a rotation counter permitted objective measurement of nocturnal activity of these animals. Compared to mock-infected hamsters and preinfection activity levels, SARS-CoV infected hamsters exhibited a greater than 90% reduction in activity (Roberts et al., 2008; Lamirande et al., 2008). This was the first objective measurement of clinical illness in hamsters. "
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