Transgenic wheat expressing a barley class II chitinase gene has enhanced resistance against Fusarium graminearum

Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St Paul, MN 55108, USA.
Journal of Experimental Botany (Impact Factor: 5.79). 02/2008; 59(9):2371-8. DOI: 10.1093/jxb/ern103
Source: PubMed

ABSTRACT Fusarium head blight (FHB; scab), primarily caused by Fusarium graminearum, is a devastating disease of wheat worldwide. FHB causes yield reductions and contamination of grains with trichothecene mycotoxins such as deoxynivalenol (DON). The genetic variation in existing wheat germplasm pools for FHB resistance is low and may not provide sufficient resistance to develop cultivars through traditional breeding approaches. Thus, genetic engineering provides an additional approach to enhance FHB resistance. The objectives of this study were to develop transgenic wheat expressing a barley class II chitinase and to test the transgenic lines against F. graminearum infection under greenhouse and field conditions. A barley class II chitinase gene was introduced into the spring wheat cultivar, Bobwhite, by biolistic bombardment. Seven transgenic lines were identified that expressed the chitinase transgene and exhibited enhanced Type II resistance in the greenhouse evaluations. These seven transgenic lines were tested under field conditions for percentage FHB severity, percentage visually scabby kernels (VSK), and DON accumulation. Two lines (C8 and C17) that exhibited high chitinase protein levels also showed reduced FHB severity and VSK compared to Bobwhite. One of the lines (C8) also exhibited reduced DON concentration compared with Bobwhite. These results showed that transgenic wheat expressing a barley class II chitinase exhibited enhanced resistance against F. graminearum in greenhouse and field conditions.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Key Message Transgenic Lilium lines have been generated by Agrobacterium-mediated transformation that have enhanced resistance to Botrytis cinerea as a consequence of ectopic expression of a rice chitinase gene. Abstract The production of ornamentals is an important global industry, with Lilium being one of the six major bulb crops in the world. The international trade in ornamentals is in the order of £60–75 billion and is expected to increase worldwide by 2–4 % per annum. The continued success of the floriculture industry depends on the introduction of new species/cultivars with major alterations in key agronomic characteristics, such as resistance to pathogens. Fungal diseases are the cause of reduced yields and marketable quality of cultivated plants, including ornamental species. The fungal pathogen Botrytis causes extreme economic losses to a wide range of crop species, including ornamentals such as Lilium. Agrobacterium-mediated transformation was used to develop Lilium oriental cv. ‘Star Gazer’ plants that ectopically overexpress the Rice Chitinase 10 gene (RCH10), under control of the CaMV35S promoter. Levels of conferred resistance linked to chitinase expression were evaluated by infection with Botrytis cinerea; sporulation was reduced in an in vitro assay and the relative expression of the RCH10 gene was determined by quantitative reverse transcriptase-PCR. The extent of resistance to Botrytis, compared to that of the wild type plants, showed a direct correlation with the level of chitinase gene expression. Transgenic plants grown to flowering showed no detrimental phenotypic effects associated with transgene expression. This is the first report of Lilium plants with resistance to Botrytis cinerea generated by a transgenic approach.
    Plant Cell Reports 03/2015; DOI:10.1007/s00299-015-1778-9 · 2.94 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The pepper receptor-like cytoplasmic protein kinase, CaPIK1, which mediates signalling of plant cell death and defence responses was previously identified. Here, the identification of a class IV chitinase, CaChitIV, from pepper plants (Capsicum annuum), which interacts with CaPIK1 and promotes CaPIK1-triggered cell death and defence responses, is reported. CaChitIV contains a signal peptide, chitin-binding domain, and glycol hydrolase domain. CaChitIV expression was up-regulated by Xanthomonas campestris pv. vesicatoria (Xcv) infection. Notably, avirulent Xcv infection rapidly induced CaChitIV expression in pepper leaves. Bimolecular fluorescence complementation and co-immunoprecipitation revealed that CaPIK1 interacts with CaChitIV in planta, and that the CaPIK1-CaChitIV complex is localized mainly in the cytoplasm and plasma membrane. CaChitIV is also localized in the endoplasmic reticulum. Transient co-expression of CaChitIV with CaPIK1 enhanced CaPIK1-triggered cell death response and reactive oxygen species (ROS) and nitric oxide (NO) bursts. Co-silencing of both CaChitIV and CaPIK1 in pepper plants conferred enhanced susceptibility to Xcv infection, which was accompanied by a reduced induction of cell death response, ROS and NO bursts, and defence response genes. Ectopic expression of CaPIK1 in Arabidopsis enhanced basal resistance to Hyaloperonospora arabidopsidis infection. Together, the results suggest that CaChitIV positively regulates CaPIK1-triggered cell death and defence responses through its interaction with CaPIK1. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.
    Journal of Experimental Botany 02/2015; DOI:10.1093/jxb/erv001 · 5.79 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Alleviating the crop loss due to biotic stress is the primary aim of plant biologists to achieve sustainable evergreen revolution in order to feed rapidly growing population. In nature, continuous evolution of plants while interacting with pathogens has generated a complex immune system that consists of preformed barriers and induced responses. The induced responses are further subdivided based upon the recognition of microbe-associated molecular patterns and effectors produced by pathogens; however, overlap exists between the downstream signaling pathways. In last decade, great deal of information about molecular aspects of plant–pathogen interactions has been generated which can be utilized for improving crops through genetic manipulation. Plant breeding has helped in the isolation of species-specific resistance components (R genes) from many plants. The molecular breeding techniques have also helped in pyramiding several components to a single variety, especially QTLs responsible for plant resistance, high yield, and nutritional quality. The identification of nonhost components in model plants and incorporation of genetically modified crops in our cropping system have raised hopes that nonhost resistance can be utilized for generating broad-spectrum pathogen tolerance breaking the barriers of species level resistance. This chapter describes the recent molecular aspects of plant–pathogen interactions focusing on the nonhost resistance components. Additionally, strategies like specific regulation of induced defense responses, manipulation of susceptibility factors, and host-induced gene silencing (HIGS) are discussed. The development of GM crops using such strategies will help in generating higher yields against pathogen infestations
    Plant Acclimation to Environmental Stress, Edited by Narendra Tuteja, Sarvajeet Singh Gill, 01/2013: chapter Chapter 16 Plant Pathogen Interactions: Crop Improvement Under Adverse Conditions: pages 433-459; Springer Science+Business Media New York., ISBN: 978-1-4614-5000-9, 978-1-4614-5001-6 (eBook)

Full-text (2 Sources)

Available from
Jun 4, 2014