Article

NADPH oxidase activation is required for migration by LIGHT in human monocytes.

Cardiovascular Medical Research Center and Department of Prescriptionology, University of Dongguk, Gyeongju City, Gyeongbuk 780-714, Republic of Korea.
Biochemical and Biophysical Research Communications (impact factor: 2.48). 08/2008; 371(4):834-40. DOI:10.1016/j.bbrc.2008.04.184 pp.834-40
Source: PubMed

ABSTRACT Homologous to lymphotoxins, shows inducible expression, and competes with herpes simplex virus (HSV) glycoprotein D (gD) for herpes virus entry mediator (HVEM; TR2) (LIGHT), a ligand of herpes virus entry mediator (HVEM), increased reactive oxygen species (ROS) and enhanced the destruction of bacteria in human monocytes. In this study, rhLIGHT was found to increase the expression of the chemokine receptors, chemokine receptor 1 (CCR1) and CCR2, as well as to accelerate the migration activity of human monocytes. Additionally, rhLIGHT was found to increase ROS via NADPH oxidase p47(phox) phosphorylation, which was found to be required for LIGHT-induced NF-kappaB activation, CCR1 and CCR2 expression, migration and IL-8 and TNF-alpha production. Taken together, these results indicate that NADPH oxidase activation is required for rhLIGHT-induced migration in human monocytes.

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Keywords

CCR1
 
CCR2
 
CCR2 expression
 
chemokine receptor 1
 
chemokine receptors
 
herpes simplex virus
 
herpes virus entry mediator
 
HSV
 
human monocytes
 
inducible expression
 
LIGHT-induced NF-kappaB activation
 
lymphotoxins
 
migration activity
 
NADPH oxidase activation
 
NADPH oxidase p47(phox)
 
reactive oxygen species
 
rhLIGHT
 
rhLIGHT-induced migration
 
TNF-alpha production
 

Sook-Kyoung Heo