Article

Influence of mobile phase composition on surface diffusion in reversed-phase liquid chromatography.

Graduate School of Science and Engineering for Research, University of Toyama, 3190, Gofuku, Toyama 930-8555, Japan.
Journal of Chromatography (impact factor: 4.53). 07/2008; 1194(2):184-91. DOI:10.1016/j.chroma.2008.04.054 pp.184-91
Source: PubMed

ABSTRACT Influence of column temperature and mobile phase composition (phi) on surface diffusion was studied in reversed-phase liquid chromatography using a C(18)-silica gel column and methanol-water mixtures. The temperature dependence of surface diffusion coefficient (D(s)) was explained by considering that of the molecular diffusivity, suggesting the presence of a kind of correlation between surface and molecular diffusions. The influence of phi on D(s) was accounted for by considering the restriction energy (E(r)) for surface diffusion. Physical meanings of the linear correlation between E(r) and the enthalpy change due to sample retention are discussed in connection with the mechanism of surface diffusion.

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Keywords

C(18)-silica gel column
 
column temperature
 
enthalpy change
 
methanol-water mixtures
 
mobile phase composition
 
molecular diffusions
 
molecular diffusivity
 
Physical meanings
 
reversed-phase liquid chromatography
 
sample retention
 

Kanji Miyabe