Membrane traffic and polarization of lipid domains during cytokinesis

Institut Curie and CNRS, UMR 144, 26 rue d'Ulm, 75248 Cedex 05, Paris, France. arnaud@
Biochemical Society Transactions (Impact Factor: 3.19). 07/2008; 36(Pt 3):395-9. DOI: 10.1042/BST0360395
Source: PubMed


Growing evidence indicates that membrane traffic plays a crucial role during the late post-furrowing steps of cytokinesis in animal cells. Indeed, both endocytosis and exocytosis contribute to stabilizing the intercellular bridge that connects the daughter cells and to the final abscission in diverse organisms. The need for several intracellular transport routes probably reflects the complex events that occur during the late cytokinesis steps such as local remodelling of the plasma membrane composition, removal of components required for earlier steps of cytokinesis and membrane sealing that leads to daughter cell separation. In this mini-review, I will focus on recent evidence showing that endocytic pathways, such as the Rab35-regulated recycling pathway, contribute to the establishment of a PtdIns(4,5)P(2) lipid domain at the intercellular bridge which is involved in the localization of cytoskeletal elements essential for the late steps of cytokinesis. Possible cross-talk between Rab35 and other endocytic pathways involved in cytokinesis are also discussed.

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    • "In addition, PtdIns(4,5)P 2 controls the recruitment of many proteins at clathrin-coated pits and regulates F-actin dynamics during endocytosis. As both exocytosis and endocytosis are essential for cytokinesis [Albertson et al., 2005; Echard, 2008; Montagnac et al., 2008; Prekeris and Gould, 2008; Neto et al., 2011], "
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    ABSTRACT: Phosphoinositides [Phosphatidylinositol (PtdIns), phosphatidylinositol 3-monophosphate (PtdIns3P), phosphatidylinositol 4-monophosphate (PtdIns4P), phosphatidylinositol 5-monophosphate (PtdIns5P), phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P(2) ), phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P(2) ), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2) ), and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P(3) )] are lowly abundant acidic lipids found at the cytosolic leaflet of the plasma membrane and intracellular membranes. Initially discovered as precursors of second messengers in signal transduction, phosphoinositides are now known to directly or indirectly control key cellular functions, such as cell polarity, cell migration, cell survival, cytoskeletal dynamics, and vesicular traffic. Phosphoinositides actually play a central role at the interface between membranes and cytoskeletons and contribute to the identity of the cellular compartments by recruiting specific proteins. Increasing evidence indicates that several phosphoinositides, particularly PtdIns(4,5)P(2) , are essential for cytokinesis, notably after furrow ingression. The present knowledge about the specific phosphoinositides and phosphoinositide modifying-enzymes involved in cytokinesis will be first presented. The review of the current data will then show that furrow stability and cytokinesis abscission require that both phosphoinositide production and hydrolysis are regulated in space and time. Finally, I will further discuss recent mechanistic insights on how phosphoinositides regulate membrane trafficking and cytoskeletal remodeling for successful furrow ingression and intercellular bridge abscission. This will highlight unanticipated connections between cytokinesis and enzymes implicated in human diseases, such as the Lowe syndrome. © 2012 Wiley Periodicals, Inc.
    Cytoskeleton 11/2012; 69(11). DOI:10.1002/cm.21067 · 3.12 Impact Factor
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    • "At this point it is unclear what exactly caused plasma membrane resident lipids to relocate into distinct poles. Polarizations of lipid domains are reported for several biological systems across species, especially during cytokinesis [61]. Experiments in fission yeast (Schizosaccharomyces pombe) revealed that there are sterols enriched at the growing cell tips and at sites of cytokinesis [62]. "
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    ABSTRACT: Background Sterols and Sphingolipids form lipid clusters in the plasma membranes of cell types throughout the animal and plant kingdoms. These lipid domains provide a medium for protein signaling complexes at the plasma membrane and are also observed to be principal regions of membrane contact at the inception of infection. We visualized different specific fluorescent lipophilic stains of the both sphingolipid enriched and non-sphingolipid enriched regions in the plasma membranes of live protoplasts of Arabidopsis thaliana. Results Lipid staining protocols for several fluorescent lipid analogues in plants are presented. The most emphasis was placed on successful protocols for the single and dual staining of sphingolipid enriched regions and exclusion of sphingolipid enriched regions on the plasma membrane of Arabidopsis thaliana protoplasts. A secondary focus was placed to ensure that these staining protocols presented still maintain cell viability. Furthermore, the protocols were successfully tested with the spectrally sensitive dye Laurdan. Conclusion Almost all existing staining procedures of the plasma membrane with fluorescent lipid analogues are specified for animal cells and tissues. In order to develop lipid staining protocols for plants, procedures were established with critical steps for the plasma membrane staining of Arabidopsis leaf tissue and protoplasts. The success of the plasma membrane staining protocols was additionally verified by measurements of lipid dynamics by the fluorescence recovery after photobleaching technique and by the observation of new phenomena such as time dependent lipid polarization events in living protoplasts, for which a putative physiological relevance is suggested.
    Plant Methods 08/2012; 8(1):28. DOI:10.1186/1746-4811-8-28 · 3.10 Impact Factor
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    • "The previous sections highlight just a few examples of what we are beginning to learn about the diverse roles of phosphoinositides during development. The list of developmental processes that may depend upon regulated phosphatidylinositol metabolism can be expanded to include neurite extension and neuronal development, angiogenesis (Im and Kazlauskas, 2006), cytokinesis (Echard, 2008; Field et al., 2005; Wong et al., 2005), cell cycle regulation (Ho et al., 2008), and flagellar biogenesis (Wei et al., 2008) to name a few. Given their integral roles during development , it is not surprising that the misregulation of PIPs and PIP binding proteins has been implicated in a growing number of diseases and developmental disorders. "
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    ABSTRACT: Phosphoinositides function as signaling precursors as well as regulators and scaffolds of signaling molecules required for important cellular processes such as membrane trafficking. Although a picture of the biochemical and cell biological functions of phosphoinositides is emerging, less is known about how these functions impact signaling on a broader scale during development. This review summarizes recent work on the role of phosphoinositides in developmental signaling and in a number of diseases and developmental disorders.
    Developmental Cell 02/2009; 16(1):12-20. DOI:10.1016/j.devcel.2008.12.006 · 9.71 Impact Factor
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