Article

Production of (S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase.

Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
Journal of Bioscience and Bioengineering (impact factor: 1.79). 05/2008; 105(4):429-31. DOI:10.1263/jbb.105.429
Source: PubMed

ABSTRACT (S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides. We constructed a system for asymmetric reduction of 2-chloroacrylate to produce (S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/l (S)-2-chloropropionate in more than 99.9%e.e.

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Keywords

2-haloacrylate reductase
 
asymmetric reduction
 
phenoxypropionic acid herbicides
 
recombinant Escherichia coli cells
 
synthetic intermediate
 
WS
 

Atsushi Kurata