Damage effects induced by electrically generated negative air ions in Caenorhabditis elegans.
ABSTRACT Electrically generated negative air ions (ENIs) have been widely used to improve indoor air quality. However, the effects of ENIs reported so far were inconsistent due to the variance in test systems, end points detected and the exposure methods. In this study, a simple model organism, the nematode Caenorhabditis elegans, was used as an in vivo system to assess the biological effects of continuous ENIs exposure. The worms were exposed to ENIs in a 10(5) 10(5) ions/cm(3) chamber and their development period, lifespan, brood size and germline cell apoptotisis were examined. The results showed that ENIs decreased the development period, shortened the lifespan, increased the germline cell apoptosis and reduced the brood size, suggesting that persistent ENIs exposure might induce damage in C. elegans. To further scrutinize the mechanisms underlying these damage effects, a reactive oxygen species (ROS) sensitive C. elegans, mev-1(kn1) mutant, and sod-3::gfp transgenic strains were used. The results showed that the persistent ENIs exposure significantly shortened the lifespan of mev-1(kn1) mutant compared to the wild type. Moreover, levels of SOD-3 were increased in an exposure time-dependent manner. Treatment with either DMSO or l-ascorbic acid, effective ROS scavengers, could rescue the upregulation of germline cell apoptosis and SOD-3 level induced by ENIs exposure, indicating that ROS may be involved in ENIs exposure-induced damaging effects.
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ABSTRACT: Variation in rates of molecular evolution has been attributed to numerous, interrelated causes, including metabolic rate, body size, and generation time. Speculation concerning the influence of metabolic rate on rates of evolution often invokes the putative mutagenic effects of oxidative stress. To isolate the effects of oxidative stress on the germline from the effects of metabolic rate, generation time, and other factors, we allowed mutations to accumulate under relaxed selection for 125 generations in two strains of the nematode Caenorhabditis elegans, the canonical wild-type strain (N2) and a mutant strain with elevated steady-state oxidative stress (mev-1). Contrary to our expectation, the mutational decline in fitness did not differ between N2 and mev-1. This result suggests that the mutagenic effects of oxidative stress in C. elegans are minor relative to the effects of other types of mutations, such as errors during DNA replication. However, mev-1 MA lines did go extinct more frequently than wild-type lines; some possible explanations for the difference in extinction rate are discussed.Genetics 12/2011; 189(4):1439-47. DOI:10.1534/genetics.111.133660 · 4.87 Impact Factor
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ABSTRACT: Negative air ions (NAI) are capable of evoking a wide range of responses in bacteria, protozoa, higher plants, insects and animals, as well as in humans. However, their precise mechanism of action is uncertain. There is evidence to show that NAI are able to influence mood, behaviour, and performance of certain tasks. In this study, rats were put into two groups, a control and an experimental group. Animals in the experimental group were exposed to an atmosphere enriched with NAI for four weeks. At the end of the experiment, we determined the blood glutathione (GSH), malondialdehyde (MDA), ceruloplasmin, hemoglobin and packed cell volume (PCV) concentrations of the rats. MDA concentrations in the experimental group were lower than that of the control animals (4.02 mu mol/l and 5.11 mu mol/l, respectively). Likewise, in the experimental animals, ceruloplasmin levels were also reduced from 33.18 mg/dl to 26.27 mg/dl, whereas no alterations in hemoglobin or glutathione concentrations were detected. An increase from 43.80% to 46.10 % was shown in the PCV content of the experimental rats. In addition, the mean weight gain of the experimental group was less than that of the control group. However, in terms of these parameters, we were unable to detect any significant differences between the two groups. In conclusion, contrary to some in vitro studies, this in vivo animal study suggests that NAI has limited or no effect upon certain biochemical parameters.Ekoloji 05/2010; 19(75):15-19. DOI:10.5053/ekoloji.2010.752 · 0.71 Impact Factor