Effective connectivity profile: A structural representation that evidences the relationship between protein structures and sequences
ABSTRACT The complexity of protein structures calls for simplified representations of their topology. The simplest possible mathematical description of a protein structure is a onedimensional profile representing, for instance, buriedness or secondary structure. This kind of representation has been introduced for studying the sequence to structure relationship, with applications to fold recognition. Here we define the effective connectivity profile (EC), a network theoretical profile that selfconsistently represents the network structure of the protein contact matrix. The EC profile makes mathematically explicit the relationship between protein structure and protein sequence, because it allows predicting the average hydrophobicity profile (HP) and the distributions of amino acids at each site for families of homologous proteins sharing the same structure. In this sense, the EC provides an analytic solution to the statistical inverse folding problem, which consists in finding the statistical properties of the set of sequences compatible with a given structure. We tested these predictions with simulations of the structurally constrained neutral (SCN) model of protein evolution with structure conservation, for singleand multidomain proteins, and for a wide range of mutation processes, the latter producing sequences with very different hydrophobicity profiles, finding that the ECbased predictions are accurate even when only one sequence of the family is known. The EC profile is very significantly correlated with the HP for sequencestructure pairs in the PDB as well. The EC profile generalizes the properties of previously introduced structural profiles to modular proteins such as multidomain chains, and its correlation with the sequence profile is substantially improved with respect to the previously defined profiles, particularly for long proteins. Furthermore, the EC profile has a dynamic interpretation, since the EC components are strongly inversely related with the temperature factors measured in Xray experiments, meaning that positions with large EC component are more strongly constrained in their equilibrium dynamics. Last, the EC profile allows to define a natural measure of modularity that correlates with the number of domains composing the protein, suggesting its application for domain decomposition. Finally, we show that structurally similar proteins have similar EC profiles, so that the similarity between aligned EC profiles can be used as a structure similarity measure, a property that we have recently applied for protein structure alignment. The code for computing the EC profile is available upon request writing to [email protected]
/* */, and the structural profiles discussed in this article can be downloaded from the SLOTH webserver http://www.fkp.tudarmstadt.de/SLOTH/.

 "We call this a meanfield (MF) model, because each site evolves independently but taking into account in a selfconsistent way the MF generated by the other sites. Approximating contact interaction energies with their hydrophobic component, the previous model established an explicit relationship between the average hydrophobicity of a site in a family of protein sequences and its connectivity at the structural level (Bastolla et al. 2005, 2008; Porto et al. 2005) and it was later extended to generate a substitution model (Bastolla et al. 2006). The MF model that we present here builds on that proposal, but is not explicitly based on hydrophobicity and it adopts an improved representation of the statistical mechanical model of the misfolded state (Minning et al. 2013). "
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ABSTRACT: Despite intense work, incorporating constraints on protein native structures into the mathematical models of molecular evolution remains difficult, since most models and programs assume that protein sites evolve independently whereas protein stability is maintained by interactions between sites. Here we address this problem by developing a new meanfield substitution model that generates independent sitespecific aminoacids distributions with constraints on the stabillity of the native state against both unfolding and misfolding. The model depends on a background distribution of amino acids and one selection parameter that we fix maximizing the likelihood of the observed protein sequence. The analytic solution of the model shows that the main determinant of the sitespecific distributions is the number of native contacts of the site and that the most variable sites are those with an intermediate number of native contacts. The meanfield models obtained taking into account misfolded conformations yield larger likelihood than models that only consider the native state, since their average hydrophobicity is more realistic, and they produce on the average stable sequences for most proteins. We evaluated the meanfield model with respect to empirical substitution models on 12 test datasets of different protein families. In all cases, the observed sitespecific sequence profiles presented smaller KullbackLeibler divergence from the meanfield distributions than from the empirical substitution model. Next, we obtained substitution rates combinining the meanfield frequencies with an empirical substitution model. The resulting meanfield substitution model assigns larger likelihood than the empirical model to all studied families when we consider sequences with identity larger than 0.35, plausibly a condition that enforces conservation of the native structure across the family. We found that the meanfield model performs better than other structurally constrained models with similar or higher complexity. With respect to the much more complex model recently developed by Bordner and Mittelmann, which takes into account pairwise terms in 1 the amino acid distributions and also optimizes the exchangeability matrix, our model performed worse for data with small sequence divergence but better for data with larger sequence divergence. The meanfield model has been implemented into the computer program Prot Evol that is freely available at http://ub.cbm.uam.es/software/Prot_Evol.php.Molecular Biology and Evolution 04/2015; DOI:10.1093/molbev/msv085 · 14.31 Impact Factor 
 "That is, the precise reason why operating directly on the labeled graph space yields worse results are not easy to rationalize, since both approaches (i.e., DSG454 and DSS454) operate basically on the same structural and chemicophysical information, although arranged in two different settings (i.e., respectively graph and sequence). However, as recently pointed out [6], a subset of the eigenvectors of the contact matrix provides good descriptors of the protein structure, showing strong correlation with hydrophobicity. It is worth to stress that, since the classification approach based on DSS454 effectively makes explicit use of the spectrum of the transition matrix (which is an elaboration of the contact/adjacency matrix) to define the order of the graph vertices in the sequence, such a technique is welljustified from the biological viewpoint, taking also into account chemicophysical information derived by the previously discussed statistical analysis. "
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ABSTRACT: This paper builds upon the fundamental paper by \citet{niwa2009} that provides the unique possibility to analyze the relative aggregation/folding propensity of the elements of the entire Escherichia coli (E. coli) proteome in a cellfree standardized microenvironment. The hardness of the problem comes from the superposition between the driving forces of intra and intermolecule interactions and it is mirrored by the evidences of shift from folding to aggregation phenotypes by singlepoint mutations \cite{doi:10.1021/ja1116233}. Here in this paper we apply different stateoftheart classification methods coming from the field of structural pattern recognition, with the aim to compare different representations of the same proteins of the Niwa et al. data base, going from pure sequence to chemicophysical labeled (contact) graphs. By this comparison, we are able to identify some interesting general properties of protein universe, going from the confirming of a threshold size around 250 residues (discriminating "easily foldable" from "difficultly foldable" molecules consistent with other independent data on protein domains architecture) to the relevance of contact graphs eigenvalue ordering for folding behavior discrimination and characterization of the E. coli data. The soundness of the experimental results presented in this paper is proved by the statistically relevant relationships discovered among the chemicophysical description of proteins and the developed cost matrix of substitution used in the various discrimination systems. 
 "There are two general views on how extraction should be accomplished: the indirect and direct methods. Indirect representation of protein spatial structure, is based on the widely held assumption that structural features are closely related to sequence composition [7] [8]. "
Conference Paper: Local Phase Quantization Texture Descriptor for Protein Classification.
International Conference on Bioinformatics & Computational Biology, BIOCOMP 2010, July 1215, 2010, Las Vegas Nevada, USA, 2 Volumes; 01/2010