Journal of Hazardous Materials

Discipline of Microbiology, University of KwaZulu-Natal, Private Bag X01, 3209, Pietermaritzburg, South Africa.
Journal of Hazardous Materials (Impact Factor: 4.53). 06/2008; 161(2-3):1157-65. DOI: 10.1016/j.jhazmat.2008.04.120
Source: PubMed


Bioremediation of arsenic-contaminated water could be a cost-effective process provided a cheap carbon source is used. In this work molasses was tested as a possible source of carbon for the growth of sulphate-reducing bacteria (SRB). Its elemental composition and the tolerance of SRB toward different arsenic species (As (III) and As (V)) were also investigated. Batch studies were carried out to assess the suitability of 1, 2.5 and 5 g/l molasses concentrations for SRB growth. The results indicated that molasses does support SRB growth, the level of response being dependant on the concentration. The percentage of sulphate reduction with molasses at 1, 2.5 and 5 g/l was not significantly different. However, growth on molasses was not as good as that obtained when lactate was used as carbon source. Molasses contained the heavy metals Al, As, Cu, Fe, Mn and Zn in concentrations of 0.54, 0.24, 8.7, 0.35, 11.1 and 19.7 microg/g, respectively. Arsenic tolerance, growth response and sulphate-reducing activity of the SRB were investigated using arsenite and arsenate solutions at final concentrations of 1, 5 and 20 mg/l for each species. The results revealed that very little SRB growth occurred at concentrations of 20 mg/l As(III) or As(V). At lower concentrations (1 mg/l) the SRB grew better with As(V) than with As(III). Arsenic pollution in most groundwater sources is below this level (1 mg/l).

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Available from: Mark D. Laing, Oct 10, 2015
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    • "This indicates that water soluble compounds and their homogeneous distribution in the soil profile via irrigated water may contribute to reductions in nematode numbers in amended soil (Hollis and Rodriguez-Kabana, 1966; Katase et al., 2009). It may also be hypothesized that the use of molasses could increase the number of sulfate-reducing bacteria (Teclu et al., 2009), thus generating increased levels of nematicidal hydrogen sulfide (Jacq and Fortuner, 1979; Rodriguez-Kabana et al., 1965). This is a more likely explanation than the exclusion of oxygen alone, because only the complete elimination of oxygen had an effect on rooteknot nematodes in a previous study (Van Gundy and Stolzy, 1962). "
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    ABSTRACT: A two-year field study was established in August 2008 at the USDA-ARS, U.S. Horticultural Research Laboratory in Fort Pierce, FL to examine the impact of anaerobic soil disinfestation (ASD) combined with soil solarization as an alternative to methyl bromide (MeBr) fumigation for control of plant–parasitic nematodes and introduced inoculum of soilborne plant pathogens. A complete factorial experiment in a split-split plot was established to evaluate three levels of applied initial irrigation (10, 5, or 0 cm), two levels of partially-composted poultry litter (CPL; amended or unamended), and two levels of molasses (amended or unamended) in combination with solarization in a raised-bed bell pepper-eggplant double crop production system. Untreated and MeBr controls were established in each block for comparison to ASD treatments. Survival of Phytophthora capsici inoculum, introduced prior to ASD treatment, was equal to that with MeBr and less than the untreated control for all solarized treatments regardless of applied soil amendments during both seasons. Survival of introduced inoculum of Fusarium oxysporum f. sp. lycopersici was least (and equivalent to the MeBr control) in treatments with applied molasses during the second season. While endemic plant-parasitic nematode populations were generally low throughout the first season, by the end of the second eggplant double crop, root-knot nematode (Meloidogyne incognita) populations in treatments not receiving molasses and/or CPL (solarization only) or not receiving irrigation at treatment averaged more than 200 nematodes per 100 cm3 of soil compared to an average of 10 nematodes per 100 cm3 in ASD treatments where molasses or molasses + CPL was applied and irrigated with 5 or 10 cm of water. Anaerobic soil disinfestation combined with solarization may provide an alternative to chemical soil fumigation for control of soilborne plant pathogens and plant–parasitic nematodes in Florida raised-bed vegetable production systems.
    Crop Protection 09/2012; 39:33–40. DOI:10.1016/j.cropro.2012.03.019 · 1.49 Impact Factor
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    • "Other technological activities have resulted in the generation of large quantities of aqueous effluents that contain high levels of heavy metals (Kadukov and Vircikova, 2005). The ability of sulfate reducing bacteria (SRB) to produce hydrogen sulfide and the high affinity of sulfide to react with divalent metallic cations provide an excellent option for achieving simultaneous removal of heavy metals and reduction of sulfate from wastewater (Bai et al., 2008; Jimenez-Rodriguez et al., 2009; Neculita et al., 2007; Radhika et al., 2006; Remoudaki et al., 2003; Southam et al., 1995; Teclu et al., 2009; Velasco et al., 2008). Anaerobic reduction of sulfate is the key step in the biological treatment of heavy metals, i.e. biogenic metal removal (Alvarez et al., 2007; Baskaran and Nemati, 2006), and the recent advances in molecular microbial ecology have provided a further impetus to promote biogenic metal removal (Ike et al., 2007; Remoudaki et al., 2003; Southam et al., 1995; Wang et al., 2001; Zhao et al., 2005). "
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    ABSTRACT: The effect of a sulfate reducing bacteria immobilized in polyvinyl alcohol (PVA) on simultaneous sulfate reduction and copper removal was investigated. Batch experiments were designed using central composite design (CCD) with two parameters, i.e. the copper concentration (10-100mg/L), and the quantity of immobilized SRB in culture solution (19-235 mg of VSS/L). Response surface methodology (RSM) was used to model the experimental data, and to identify optimal conditions for the maximum sulfate reduction and copper removal. Under optimum condition, i.e. approximately 138.5mg VSS/L of sulfate reducing bacteria immobilized in PVA, and approximately 51.5mg/L of copper, the maximum sulfate reduction rate was 1.57 d(-1) as based on the first-order kinetic equation. The data demonstrate that immobilizing sulfate reducing bacteria in PVA can enhance copper removal and the resistance of the bacteria towards copper toxicity.
    Bioresource Technology 02/2010; 101(12):4354-61. DOI:10.1016/j.biortech.2010.01.094 · 4.49 Impact Factor
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    ABSTRACT: Spent mushroom compost (SMC) is commonly used as a carbon source for passive treatment systems in South Korea; however, it has some drawbacks, such as sulfate release from itself. Consequently, investigations to identify effective substitutes for SMC are necessary. In this study, batch experiments were conducted for 27 days to evaluate the efficiency of rice wine waste (RWW) for reducing sulfate and removing dissolved metals within synthetic acid mine drainage (AMD). The results showed that RWW could be more suitable than SMC, which even released sulfate in the early stage of the experiment, for sulfate reduction by sulfate-reducing bacteria. Both materials produced similar results with respect to the removal of dissolved metals, such as Fe and Al. Furthermore, a mixture of SMC and RWW showed the greatest efficiency in sulfate removal. Overall, both RWW and the mixed carbon source showed comparable performance to SMC, which indicated that RWW had a great potential for use as a carbon source for AMD treatment.
    Environmental Earth Sciences 05/2013; 71(10). DOI:10.1007/s12665-013-2852-7 · 1.77 Impact Factor
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