In vitro activity of Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole against different dermatophytes

Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52., Szeged, Hungary.
Antonie van Leeuwenhoek (Impact Factor: 1.81). 07/2008; 94(3):463-70. DOI: 10.1007/s10482-008-9263-x
Source: PubMed


Strains of five dermatophyte species (Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum and Trichophyton tonsurans) were selected for testing against Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole (FCZ). Inhibition of microconidia germination and growth was detected with MICs of PAF ranging from 1.56 to 200 microg ml(-1) when it was used alone, or at constant concentration (100 microg ml(-1)) in combination with FCZ at from 0.25 to 32 microg ml(-1). The MICs for FCZ were found to be between 0.25 and 128 microg ml(-1). PAF caused a fungicidal effect at 200 microg ml(-1) and reduced growth at between 50 and 200 microg ml(-1). Total growth inhibition with fungistatic activity was detected at 64 microg ml(-1) of FCZ for M. gypseum, T. mentagrophytes, and T. tonsurans, and at 32 microg ml(-1) FCZ for M. canis and T. rubrum. PAF and FCZ acted synergistically and/or additively on all of the tested fungi except M. gypseum, where no interactions were detected.

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Available from: Laszlo Galgoczy, Apr 17, 2015
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    • "Aala and collaborators [41] found good activity between allicin in combination with ketoconazole or fluconazole demonstrating a synergistic or additive interaction against dermatophytes. Galgóczy and coauthors [42] evaluated the in vitro antifungal activity against strains of dermatophytes, combining the protein (PAF) of Penicillium chrysogenum with fluconazole (FCZ). PAF and FCZ acted synergistically and/or additively on all of the tested fungi except Microsporum gypseum, for which no interactions were detected [42]. "
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    Evidence-based Complementary and Alternative Medicine 06/2014; 2014(4). DOI:10.1155/2014/957860 · 1.88 Impact Factor
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    • "The extracellular, defensin-like antifungal proteins secreted by filamentous ascomycetes are interesting in this respect both in medicine and agriculture . These proteins have potent antifungal activity against potential human, plant and food-borne pathogenic fungal species [1] [2] [3], they could not have any toxic effects on plant and mammalian cells in vitro and in vivo [2] [4] [5], they can interact synergistically with other antifungal drugs and peptides [6] [7] [8], and they have high stability against protease degradation, high temperature and that they are active within broad pH range [1]. These facts and their low costs of production could make them promising candidates as a base of commercial biopesticides, medicines and food preservatives against harmful filamentous fungi [1] [2]. "
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    Protein Expression and Purification 11/2013; 94:79-84. DOI:10.1016/j.pep.2013.11.003 · 1.70 Impact Factor
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    • "The ␤-defensin-like antimicrobial peptides with PAF-cluster are interesting in practical respect, and their potential applications are intensively studied both in medical and agricultural fields. Their following features that they have potent antifungal activity against potential human and plant pathogenic fungal species [12], that they could not have any toxic effects on plant and mammalian cells in vitro [32] [33], that they can interact synergistically with other antifungal drugs and peptides [10] [11] [23], and their low costs of production could make them suitable as active ingredients of commercial biopesticides and medicines [21] [26]. "
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