Article

Isolation of a respiratory-deficient Kluyveromyces fragilis mutant for the production of ethanol from Jerusalem artichoke.

Laboratoire de Génétique et Microbiologie INRA-ENSA 34060 Montpellier Cedex, France.
Biotechnology and Bioengineering (impact factor: 3.95). 06/1987; 29(7):850-8. DOI:10.1002/bit.260290707 pp.850-8
Source: PubMed

ABSTRACT A respiratory-deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especially from Jerusalem artichoke extracts containing large amounts of high polyfructosans ("early" extracts).

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Keywords

anaerobic conditions
 
ethanol production
 
ethidium bromide mutagenesis
 
extracts
 
inulinase activity
 
Kluyveromyces fragilis
 
large amounts
 
respiratory-deficient
 

J P Guiraud