Bacterial adhesion to worn silicone hydrogel contact lenses.
ABSTRACT The aim of this study was to, firstly, investigate whether silicone-hydrogel contact lenses (CL) are more or less susceptible to bacterial adhesion than conventional ones and, secondly, assess the influence of lens wear in the extent of bacterial adhesion. Four silicone-hydrogel CL (galyfilcon A, balafilcon A, lotrafilcon A, and lotrafilcon B) and one conventional hydrogel (etafilcon A) CL were tested.
Bacterial adhesion experiments were performed on unworn and worn CL using the strain Staphylococcus epidermidis 9142. Worn lenses were obtained from a group of 31 subjects fitted with a silicone-hydrogel CL in one eye and a conventional hydrogel CL as contralateral pair. These lenses were used on a daily basis in combination with a multipurpose lens care solution. Adhesion assays were carried out in a parallel plate flow chamber, followed by image analysis. Hydrophobicity, roughness, and topography of the lenses surfaces were assessed through contact angle measurements and atomic force microscopy.
Unworn conventional and silicone-hydrogel CL were equally susceptible to bacterial adhesion of S. epidermidis. Conversely, worn conventional hydrogel (etafilcon A) were more prone to bacterial adhesion than worn silicone-hydrogel materials, which exhibited similar adhesion extents among them. The results also showed that the lens surface properties such as hydrophobicity, roughness, and surface topography changed during wear. The alteration of surface hydrophobicity of silicone and conventional hydrogel CL during wear had a great impact on lens bacterial adhesion susceptibility. Accordingly, balafilcon A becomes significantly less hydrophobic and less prone to bacterial adhesion after lens wear, whereas etafilcon A becomes more hydrophobic and also more susceptible to bacterial adhesion (p < 0.05).
Worn silicone-hydrogel galyfilcon A, balafilcon A, lotrafilcon A, and lotrafilcon B are equally prone to microbial adhesion of S. epidermidis and generally less susceptible than the conventional hydrogel.
Full-textDOI: · Available from: Diana Rodrigues, May 30, 2015
[Show abstract] [Hide abstract]
ABSTRACT: To investigate whether cosmetic contact lenses (CCL) with surface pigments affect microbial adherence. Fifteen brands of CCL were purchased from optical, non-optical retail outlets, and via the Internet. A standardized rub-off test was performed on each CCL (five lenses per brand) to confirm the location of the pigments. The rub-off test comprised gentle rubbing on the surfaces of each CCL with wetted cotton buds for a maximum of 20 rubs per surface. A new set of CCL (five lenses per brand) were incubated in Pseudomonas aeruginosa overnight. Viable counts of adhered bacteria were determined by the number of colony-forming units (CFU) on agar media on each lens. The adherence of P. aeruginosa as well as Staphylococcus aureus and Serratia marcescens to three brands of CCL (A-C) (five lenses per brand) were also compared to their adherences on their clear counterparts. Only two of the 15 brands of CCL tested (brands B and C) had pigments that did not detach with the rub-off test. The remaining 13 brands of CCL all failed the rub-off test and these lenses showed higher P. aeruginosa adherence (8.7×10(5)-1.9×10(6)CFU/lens). Brands B and C lenses showed at least six times less bacterial adhesion than the other 13 brands. Compared to their clear counterparts, bacterial adherence to brands B and C lenses did not differ significantly, whereas brand A lenses showed significantly higher adherence. Surface pigments on CCL resulted in significantly higher bacterial adherence.Contact lens & anterior eye: the journal of the British Contact Lens Association 01/2014; 37(4). DOI:10.1016/j.clae.2013.12.002 · 2.00 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: To describe the adhesion properties of Acanthamoeba castellanii trophozoites to silicone hydrogel contact lenses of first generation (lotrafilcon A), second generation (galyfilcon A), and third generation (comfilcon A) and correlate the results with their specific surface characteristics, time of interaction, and suspension media. Qualitative and quantitative assessments of the adhesion of 200 trophozoites of A. castellanii on contact lenses in culture medium (Bacto Casitone) and isotonic saline (IS) at different time points (15 minutes and 6 hours) were determined. By scanning electron microscopy, A. castellanii trophozoites were observed firmly adhered to the surface of hydrogel lenses after 15 minutes of interaction. The surface of lotrafilcon A lenses on which amoebae adhere better (16.4±10.2 amoebae/lens section) is rough and folded, which increases the contact surface with trophozoites, allowing acanthopodia to attach firmly. Contrarily, galyfilcon A lenses have a smoother surface, and lower numbers of amoebae were observed adhered to these lenses (4.7±2.9 amoebae/lens section). Even fewer amoebae adhered to the smoother surface of the comfilcon A lens (2.2±1.7 amoebae/lens section). Trophozoites showed similar behavior in both Bacto Casitone medium and IS. A rough surface may contribute to better adhesion of amoebae to silicone hydrogel lenses. Although a reduced numbers of trophozoites adhered to smooth lenses, trophozoites are a risk factor for amoebic keratitis. Isotonic saline facilitated trophozoite survival, suggesting that homemade saline solutions may contribute to the persistence of trophozoites, especially when there is no proper hygiene regimen used with the contact lens cases.Eye & contact lens 04/2014; DOI:10.1097/ICL.0000000000000024 · 1.68 Impact Factor