Bacterial adhesion to worn silicone hydrogel contact lenses.
ABSTRACT The aim of this study was to, firstly, investigate whether silicone-hydrogel contact lenses (CL) are more or less susceptible to bacterial adhesion than conventional ones and, secondly, assess the influence of lens wear in the extent of bacterial adhesion. Four silicone-hydrogel CL (galyfilcon A, balafilcon A, lotrafilcon A, and lotrafilcon B) and one conventional hydrogel (etafilcon A) CL were tested.
Bacterial adhesion experiments were performed on unworn and worn CL using the strain Staphylococcus epidermidis 9142. Worn lenses were obtained from a group of 31 subjects fitted with a silicone-hydrogel CL in one eye and a conventional hydrogel CL as contralateral pair. These lenses were used on a daily basis in combination with a multipurpose lens care solution. Adhesion assays were carried out in a parallel plate flow chamber, followed by image analysis. Hydrophobicity, roughness, and topography of the lenses surfaces were assessed through contact angle measurements and atomic force microscopy.
Unworn conventional and silicone-hydrogel CL were equally susceptible to bacterial adhesion of S. epidermidis. Conversely, worn conventional hydrogel (etafilcon A) were more prone to bacterial adhesion than worn silicone-hydrogel materials, which exhibited similar adhesion extents among them. The results also showed that the lens surface properties such as hydrophobicity, roughness, and surface topography changed during wear. The alteration of surface hydrophobicity of silicone and conventional hydrogel CL during wear had a great impact on lens bacterial adhesion susceptibility. Accordingly, balafilcon A becomes significantly less hydrophobic and less prone to bacterial adhesion after lens wear, whereas etafilcon A becomes more hydrophobic and also more susceptible to bacterial adhesion (p < 0.05).
Worn silicone-hydrogel galyfilcon A, balafilcon A, lotrafilcon A, and lotrafilcon B are equally prone to microbial adhesion of S. epidermidis and generally less susceptible than the conventional hydrogel.
Full-textDOI: · Available from: Diana Rodrigues, Aug 12, 2015
- SourceAvailable from: Lyndon W Jones
[Show abstract] [Hide abstract]
- "Immediately after being placed on the eye, contact lenses are coated with a protein layer and most proteins attach strongly to the material, with typically less than 50% being removed by conventional care regimens   . The deposition of certain proteins to contact lenses has shown to increase the risk of microbial cell attachment to the lens material   , and is also associated with inflammatory complications such as giant papillary conjunctivitis  "
ABSTRACT: Proteins are a key component in body fluids and adhere to most biomaterials within seconds of their exposure. The tear film consists of more than 400 different proteins, ranging in size from 10 to 2360 kDa, with a net charge of pH 1-11. Protein deposition rates on poly-2-hydroxyethyl methacrylate (pHEMA) and silicone hydrogel soft contact lenses have been determined using a number of ex vivo and in vitro experiments. Ionic, high water pHEMA-based lenses attract the highest amount of tear film protein (1300 μg/lens), due to an electrostatic attraction between the material and positively charged lysozyme. All other types of pHEMA-based lenses deposit typically less than 100 μg/lens. Silicone hydrogel lenses attract less protein than pHEMA-based materials, with <10 μg/lens for non-ionic and up to 34 μg/lens for ionic materials. Despite the low protein rates on silicone hydrogel lenses, the percentage of denatured protein is typically higher than that seen on pHEMA-based lenses. Newer approaches incorporating phosphorylcholine, polyethers or hyaluronic acid into potential contact lens materials result in reduced protein deposition rates compared to current lens materials.Contact lens & anterior eye: the journal of the British Contact Lens Association 04/2012; 35(2):53-64. DOI:10.1016/j.clae.2011.12.005 · 2.00 Impact Factor