Article

Therapeutic application of RNA interference against foot-and-mouth disease virus in vitro and in vivo.

Foreign Animal Disease Division, National Veterinary Research and Quarantine Service, Manan-Gu, Anyang, Gyeonggi-Do, Republic of Korea.
Antiviral research (impact factor: 3.61). 08/2008; 80(2):178-84. DOI:10.1016/j.antiviral.2008.06.001 pp.178-84
Source: PubMed

ABSTRACT Foot-and-mouth disease (FMD) is an economically important animal disease because of the speed of its transmission. Routine vaccination may not be effective for early protection in an outbreak situation. Small interfering RNA (siRNA) can be used in a rapid and effective antiviral approach. However, siRNA has limitations when used in disease prevention, such as a short duration of action. In this study, we have demonstrated that treatment with siRNA after FMD virus (FMDV) infection has an antiviral effect and could be effective in control of FMDV. We applied adenoviruses expressing siRNA both before and after FMDV infection in vitro and in vivo. Treatment after FMDV infection gave effective viral inhibition, but a combination of treatment before and after FMDV infection gave the best results in IBRS-2 cells. We obtained high survival rates in suckling mice by the use of therapeutic injections following challenge. The results of this study suggest that treatment with siRNA could enhance antiviral effects and may be helpful in the control of FMDV in an outbreak.

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Keywords

adenoviruses
 
antiviral effect
 
antiviral effects
 
disease prevention
 
effective antiviral approach
 
effective viral inhibition
 
FMDV
 
FMDV infection
 
Foot-and-mouth disease
 
IBRS-2 cells
 
outbreak situation
 
Routine vaccination
 
short duration
 
siRNA
 
suckling mice
 
survival rates
 
therapeutic injections
 
vivo