Microarray expression profiling of Arabidopsis thaliana L. in response to allelochemicals identified in buckwheat. J Exp Bot

Biodiversity Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kan-non-dai, Tsukuba, Ibaraki, 305-8604 Japan.
Journal of Experimental Botany (Impact Factor: 5.53). 08/2008; 59(11):3099-109. DOI: 10.1093/jxb/ern168
Source: PubMed


Buckwheat (Fagopyrum esculentum Moench) is an important annual plant cultivated for grain or as a cover crop in many countries, and it is also used for weed suppression in agro-economic systems through its release of allelochemicals. Little is known, however, concerning the mode of action of allelochemicals or plant defence response against them. Here, microarrays revealed 94, 85, and 28 genes with significantly higher expression after 6 h of exposure to the allelochemicals fagomine, gallic acid, and rutin, respectively, compared with controls. These induced genes fell into different functional categories, mainly: interaction with the environment; subcellular localization; protein with binding function or cofactor requirement; cell rescue; defence and virulence; and metabolism. Consistent with these results, plant response to allelochemicals was similar to that for pathogens (biotic stress) or herbicides (abiotic stress), which increase the concentration of reactive oxygen species (ROS; with consequent oxidative stress) in plant cells. The data indicate that allelochemicals might have relevant functions, at least in part, in the cross-talk between biotic and abiotic stress signalling because they generate ROS, which has been proposed as a key shared process between these two stress mechanisms.


Available from: Anna Golisz
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    • "Allelopathy is characterized primarily by a negative interaction between plants through chemical signaling (El-khawas and Shehata, 2005). According to Golisz et al. (2008), allelopathy consists in the plant ability to synthetize metabolites that inhibit or stimulate the growth and development of other neighboring plants, with a competition for limited resources as light, water and nutrients. The allelochemicals found in soil cover plants are soluble in water, and are released in the environment by volatilization, radicular exudation, lixiviation and plant tissue breakdown. "

    African journal of agricultural research 12/2015; 10(46):4215-4221. DOI:10.5897/AJAR2015.10413 · 0.26 Impact Factor
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    • "GSH provides the most important intracellular defense against ROS (Mullineaux and Rausch 2005). It also plays a central role in detoxification of xenobiotics as well as the expression of stressresponsive genes (Ding et al. 2007; Golisz et al. 2008; Sytykiewicz 2011). Though the concentration of GSSG in both annual Medicago species significantly decreased, GSH concentration and consequently the ratio of GSH/ GSSG were significantly increased in both annual Medicago species in response to juglone exposure. "
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    ABSTRACT: The current study was conducted to evaluate the physiological and molecular response of self-regenerating annual Medicago species (M. polymorpha and M. lupulina) to juglone exposure. A randomized complete block design was performed in which two treatment groups consisted of a control and juglone (10-4 M) allotted to main plots and genotypes assigned to subplots. A significant increase in the concentration of GSH and the GSH/ GSSG ratio was observed in both annual Medicago species in response to juglone exposure. However, such response was greater in M. lupulina than M. polymorpha. The activity of all antioxidant enzymes (CAT, APX, GST, and GPOX) was significantly increased by juglone. In response to juglone, the expression of WRKY was significantly decreased. The transcription of CBF4, Zpt2-2, CAT, and GST genes was highly induced by juglone in annual Medicago species. Higher expression of CBF4, Zpt2-2, CAT and GST genes in M. lupulina which showed more juglone-tolerance can be associated with higher tolerance against allelochemical stress. It can be concluded that M. polymorpha is juglone-sensitive because it started to show chlorosis of leaves after a week subjecting to juglone. Though M. lupulina seems to be more juglone-tolerant at least in short term, long-term exposure of juglone should also be examined to find out its suitability to grow in walnut orchards. Considering the presence of cross-talk between different stresses, we can propose that M. lupulina is more tolerant to other abiotic stresses compared to M. polymorpha.
    Acta Physiologiae Plantarum 11/2015; 37(11). DOI:10.1007/s11738-015-1999-0 · 1.58 Impact Factor
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    • "The mode of action of phenolic allelochemicals has been investigated by biochemical and genetic experiments. When Arabidopsis was exposed to gallic acid, the synthesis of several ROS-related gene products was increased (Golisz et al., 2008). Some phenolic acids, including gallic acid, caused a slight inhibition of phosphate uptake and a depolarization of the membrane potentials of barley (Hordeum vulgare) roots. "
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    ABSTRACT: Common buckwheat (Fagopyrum esculentum) is well known for its weed-suppressive ability. This property is probably due to multiple factors such as resource competition, allelopathy and soil property modifications. A better understanding of the mechanisms of weed suppression could improve the development of new strategies for weed management using cover crops. In this review, the different factors that could be potentially responsible for weed suppression by common buckwheat are discussed. Special emphasis is put on the role of allelopathy.
    Environmental Control in Biology 01/2015; 53(1):1-6. DOI:10.2525/ecb.53.1
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