Article

Microarray expression profiling of Arabidopsis thaliana L. in response to allelochemicals identified in buckwheat

Biodiversity Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kan-non-dai, Tsukuba, Ibaraki, 305-8604 Japan.
Journal of Experimental Botany (Impact Factor: 5.79). 08/2008; 59(11):3099-109. DOI: 10.1093/jxb/ern168
Source: PubMed

ABSTRACT Buckwheat (Fagopyrum esculentum Moench) is an important annual plant cultivated for grain or as a cover crop in many countries, and it is also used for weed suppression in agro-economic systems through its release of allelochemicals. Little is known, however, concerning the mode of action of allelochemicals or plant defence response against them. Here, microarrays revealed 94, 85, and 28 genes with significantly higher expression after 6 h of exposure to the allelochemicals fagomine, gallic acid, and rutin, respectively, compared with controls. These induced genes fell into different functional categories, mainly: interaction with the environment; subcellular localization; protein with binding function or cofactor requirement; cell rescue; defence and virulence; and metabolism. Consistent with these results, plant response to allelochemicals was similar to that for pathogens (biotic stress) or herbicides (abiotic stress), which increase the concentration of reactive oxygen species (ROS; with consequent oxidative stress) in plant cells. The data indicate that allelochemicals might have relevant functions, at least in part, in the cross-talk between biotic and abiotic stress signalling because they generate ROS, which has been proposed as a key shared process between these two stress mechanisms.

0 Bookmarks
 · 
108 Views
  • Reactive Oxygen and Nitrogen Species Signaling and Communication in Plants Volume 23, First edited by Kapuganti Jagadis Gupta, Abir U. Igamberdiev, Series Editor Frantisˇek Balusˇka, 01/2015: chapter Interaction of Calcium Signalling with Reactive Oxygen and Reactive Nitrogen Species: pages 301-316; Springer Cham Heidelberg New York Dordrecht London., ISBN: ISSN 1867-9056 (electronic); ISBN 978-3-319-10078-4; ISBN 978-3-319-10079-1 (eBook)
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study developed and validated a simple method to study allelopathic effects of belowground root interactions between two plant species. The objective was to separate resource competition for water, nutrients and light from phytotoxic root interactions between an allelopathic donor plant (cover crop) and a sensitive receiver plant (annual weed). This was achieved by conducting experiments in non-sterile soil in pots under controlled conditions in a phytotron. Water and nutrient supply were kept constant in sufficient amounts throughout the experiment. The effect of shading was evaluated by the presence or absence of vertical nets between the competing plants. Growth repressing allelopathic root interactions were studied by separating the rhizospheres of the weed and the cover crop species with impenetrable plastic barriers in one treatment and by allowing interplant root contacts in the other treatment. We found that the growth repressing effect of Fagopyrum esculentum (common buckwheat) and Sorghum bicolor x Sorghum sudanense (sorghum sudangrass hybrid) on Amaranthus retroflexus (redroot pigweed) was very strong (91% and 68% respectively) and that it was due to both competitive shading effects (64% and 21% respectively) and root interactions (75% and 60% respectively) of potentially allelopathic nature.
    Allelopathy Journal 01/2014; 34(2):227-240. · 0.58 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Allelopathy phenomenon describes both positive and negative interactions between plants through chemicals released into environment. Inhibition of growth of various organs and delay or restriction of seed germination are most frequently observed effects of toxicity of allelochemicals, although some of them, when applied in minimal concentrations, lead to the effect of hormesis (stimulation of seed germination or seedling growth). Plants subjected to some allelopathic compounds respond by induction of oxidative stress, manifested as overproduction of reactive oxygen species (ROS), alterations in cellular antioxidant system (activity of catalase, superoxide dismutase, and enzymes of Halliwell–Asada–Foyer cycle, the level of ascorbate and glutathione). Due to differences in plant sensitivity to allelochemicals, and considering a wide spectrum of concentration of already tested compounds, it is hardly to believe that induction of oxidative stress is a global and uniform reaction to all allelopathins. There are several examples of allelopathic compounds that act as antioxidant rather then prooxidant agents. In the chapter we present the data describing induction of alterations in ROS production or/and metabolism in acceptor plants by various allelochemicals or extracts of other plants releasing allelochemicals into environment. Special attention is paid on oxidative deterioration induced by essential oils. Accumulation of ROS and modification of growth and/or development of organs of acceptor plants as induced by allelotoxins were correlated with “stress induced morphogenic responses” (SIMRs), modification of phytohormonal balance and factors crucial for cell wall formation. It was proposed a link between phytotoxicity of allelochemicals and activation of ROS-mediated transduction pathways, involving also calcium dependent intracellular signaling.
    Reactive Oxygen and Nitrogen Species Signaling and Communication in Plants, Signaling and Communication in Plants 23,, Edited by K.J. Gupta, A.U. Igamberdiev, 01/2015: chapter Allelopathic Compounds as Oxidative Stress Agents: Yes or NO: pages 155-176; Springer International Publishing Switzerland., ISBN: 978-3-319-10078-4

Full-text (2 Sources)

Download
82 Downloads
Available from
May 21, 2014