[Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique].
ABSTRACT To explore the appropriate amount of template DNA for Sinofiler Kit.
The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.
It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.
Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.