Toxicity assessment of the puffer fish Lagocephalus lagocephalus from the Tunisian coast.
ABSTRACT This study was undertaken to assess the risk of poisoning due to consumption of the puffer fish Lagocephalus lagocephalus collected along the Tunisian coast. Wistar rats were daily intraperitoneally injected, for 10 days, with acidic extracts of liver or flesh (muscles + skin) of L. lagocephalus. Control rats received injections of NaCl (0.9%). No mortality and no evident signs of neurotoxicity were recorded in treated rats. Conversely, treatment led to: (1) diarrhoea and body and organ (liver, kidney) weights loss; (2) oxidative stress evidenced by an increase in lipid peroxidation (TBARS) and conversely a decrease in antioxidant enzyme activities (SOD, catalase, GSH-Px) in tissues (blood cells, liver, kidneys); (3) a decrease in alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in blood plasma.
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ABSTRACT: Abstract We examined whether the levels of oxidative stress biomarkers measured in blood reflect the tissue redox status. Data from studies that measured redox biomarkers in blood, heart, liver, kidney and skeletal muscle were analyzed. In seven out of nine investigated redox biomarkers (malondialdehyde, reduced glutathione, superoxide dismutase, catalase, glutathione peroxidase, vitamin C and E) there was generally good qualitative and quantitative agreement between the blood and tissues. In contrast, oxidized glutathione and the reduced to oxidized glutathione ratio showed poor agreement between the blood and tissues. This study suggests that most redox biomarkers measured in blood adequately reflect tissue redox status.Biomarkers 01/2015; DOI:10.3109/1354750X.2014.1002807 · 2.52 Impact Factor
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ABSTRACT: Abstract Several dietary strategies to ameliorate poorer growth observed to occur at temperatures above the upper thermal optima were examined with juvenile barramundi (Lates calcarifer). A reference (REF) and three experimental diets, one with an increased protein to energy ratio (PRO), another with an increased level of the amino acid histidine (HIS) and a third with supplementation of dietary nucleotides (NUC), were each fed to fish at either 30 °C or 37 °C for a 28-day period. Growth was affected by both temperature and diet. Fish fed the PRO diet at 30 °C grew fastest, but not faster than those fed the NUC diet at the same temperature. The addition of the amino acid histidine to the diet did not improve growth rates at either temperature. At water temperatures of 37 °C, only the fish fed the PRO diet had growth rates equivalent to those of fish at the 30 °C temperatures. Other key factors including feed intake, feed conversion rate, nutrient and energy retention and plasma enzymology were also all affected by temperature and diet. This study shows that the use of a diet with an increased protein to energy ratio provides significant benefits in terms of reducing the impact of growth retardation at higher temperatures.Aquaculture Nutrition 08/2009; 16(4). DOI:10.1111/j.1365-2095.2009.00670.x · 1.67 Impact Factor
Article: Tetrodotoxin: a potent marine toxin[Show abstract] [Hide abstract]
ABSTRACT: Tetrodotoxin (TTX) is a neurotoxin found in puffer fish and other marine animals. This toxin is predominantly isolated from the skin, viscera, ovaries, and liver of the puffer fish. The toxin is produced by various species of bacteria, and TTX-bearing animals may have absorbed and accumulated it through the food chain. TTX is widely used in many laboratories as an important pharmacological reagent because of its ability to selectively block the sodium channels on the nerve membrane. No antidote is available for clinical use. The mainstay of treatment is careful observation and serial neurological assessment to monitor the progression of the clinical effects so that respiratory failure or cardiac effects are appropriately treated.Toxin Reviews 06/2010; 29(2):60-70. DOI:10.3109/15569543.2010.487631 · 0.84 Impact Factor