Cardioprotective effects and mechanism of action of Polyphenols extracted from Propolis against Doxorubicin toxicity

Laboratoire de Toxicologie Moléculaire, Faculté des Sciences, Université de Jijel, Jijel, Algeria.
Pakistan journal of pharmaceutical sciences (Impact Factor: 0.68). 08/2008; 21(3):201-9.
Source: PubMed


Propolis is one of the major hive products of bees and is rich in flavonoids, which are known for antioxidant activities. It is well known that the chemical properties of phenolic acids or flavonoids, in terms of the availability of the phenolic hydrogens as hydrogen donating radical scavengers, predict their antioxidant properties. In this study, the flavonoids scavenging activity of propolis has been exploited to obtain protection against the peroxidative damage in rat heart mitochondria which was induced by the administration of an acute dose of doxorubicin (20 mg kg(-1), i.p). The peroxidative lesions were evaluated biochemically and biophysically, 24 H after DXR administration. Abnormal biochemical changes in heart mitochondria from DXR treated rats including a marked increase in both malondialdehyde (MDA) and anion superoxide production; decrease both of respiratory chain ratio (RCR= V3/V4) and P/O. Pretreatment of rats with propolis extract, given per os (100 mg/kg/day) during four days prior to DXR injection, substantially reduced the peroxidative damage in the heart mitochondria: we showed significant reducing both of mitochondrial MDA formation and production of superoxide anion, restoration of RCR and P/O and reducing of rate and the amplitude of mitochondrial swelling. The data demonstrate that antioxidants from natural sources may be useful in the protection of cardiotoxicity in patients who receive doxorubicin and as reported for its claimed beneficial effect on human health by biomedical literature.

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    • "The effects of propolis in maintaining the mitochondrial and cellular GSH might be due to direct neutralysing free- radicals, or increasing of GSH synthesis by propolisbioactive compounds. Indeed, recent study showed that propolis at 100 mg/kg prevents doxorubicin cardiotoxicity by the improvement of intracellular and serum GSH rates (Alyane et al., 2008 "
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    ABSTRACT: Doxorubicin (DOX) is a potent anticancer drug; its use has been limited by its hepatotoxicity, which is due to free radicals generation. Propolis, a honeybee product very rich in flavonoids and therapeutic possibilities, has gained popularity as a food and alternative medicine. The present study treats DOX pro-oxidant effect on hepatic cells and mitochondrial functions. The prophylactic effect of propolis ethanolic extract (EEP) against DOX induced mitochondrial oxidative stress has also been investigated. We find that doxorubicin at the amount of 10mg/Kg altered liver mitochondrial functions as attested by the overproduction of superoxide anion (O2(2-)) by mitochondrial respiratory chain complex III. The hepatic tissue from DOX treated rats showed also a marked depletion in reduced GSH contents and an inhibition in (Mn-SOD), (Cu-Zn SOD) and (CAT) enzymatic activities. DOX increase cytosolic and mitochondrial lipid peroxidation as attested by the MDA content. These results are reversed after one mount per os pretreatment by EEP at the amount of 100mg/kg. Propolis polyphenolic fraction protects liver tissue from oxidative stress by protecting mitochondrial functions and reinforcement of enzymatic and non enzymatic antioxidants.
    Pakistan journal of pharmaceutical sciences 11/2014; 27(6):1891-1897. · 0.68 Impact Factor
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    • "HPLC analysis revealed the presence of most important flavonol (quercetin, myricetin, kaempferol), flavanole (catechin) and phenolic acids (gallic acid, P. coumaric, ferulic acid) in significantly higher concentrations. Many studies supported the cardioprotective effect of plant polyphenols (Alyane et al., 2008). Flavonoids can show their cardioprotective potential by various mechanisms. "
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    ABSTRACT: The objective of this study was to determine the comparative cardioprotective potential of bark and gemmomodified extract of Terminalia arjuna. Cardioprotective potential was evaluated against isoproterenol induced cardiotoxicity. Both ways of treatment, curative and preventive were studied. In preventive cardioprotective potential, rabbits were pretreated with both extracts of T. arjuna (200 mg/kg) for three weeks and then cardiotoxicity was induced with isoproterenol. In curative way of treatment isoproterenol was given for two days and then these cardio intoxicated rabbits were treated with plant extracts. The activities of cardiac marker enzymes (CK-MB, AST, ALT, and LDH) and antioxidants enzymes (SOD, CAT) were analyzed in serum and heart tissues of different groups of experimental rabbits. Isoproterenol significantly (P<0.001) increased the level of enzymes. The three week prior administration and curative treatment of T. arjuna extracts resorted the enzymes and antioxidants level equal to normal. HPLC analysis of the extracts confirmed the presence of important flavonol and phenolic acids in both extracts. It can be concluded that gemmomodified and bark extract of T. arjuna has strong cardioprotective potential.
    Pakistan Veterinary Journal 01/2012; 32(2). · 1.39 Impact Factor
    • "Recently, we have reported that flavonoids of propolis extract protect the liver and heart tissues in rats treated with anticancer drugs[9] and we hypothesized that the protective effect of flavonoids could be due to their capacity to capture and deactivate the free radicals. Adopting the method of DPPH (1.1, 2-Diphenyl hydrazyl), our study shows that the ethanolic extract of propolis has a very strong scavenger effect; it is more active at concentrations up to 0.12 mg/ml (reduction of 88.30% against 75.51% with quercetin at concentration 1 mg/ml). "
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    ABSTRACT: We evaluated the effects of propolis extract on renal oxidative stress induced by doxorubicin throughout an analytical and pharmacological study of the eastern Algerian propolis using thin layer chromatography, ultra-violet-high-performance liquid chromatography) and gas chromatography-mass spectrometry. The pharmacological study was carried out in vivo on Wistar rat pre-treated with propolis extract 100 mg/kg/day for seven days. Doxorubicin at 10 mg/kg of body weight was administered intravenously on Day 7. Serum creatinine concentration, scavenging effect of flavonoids, lipid peroxidation and glutathione concentration were measured. Chemical analysis allowed identification and quantification of the phenolic compounds including pinostrombin chalcone (38.91%), galangin (18.95%), naringenin (14.27%), tectochrysin (25.09%), methoxychrysin (1.14%) and a prenylated coumarin compound suberosin (1.65%). The total flavonoid concentration in the propolis extract was 370 mg (quercetin equivalents QE) /g dry weight (QE/g DWPE). Propolis extract restored the renal functions and reduced the toxic effect of doxorubicin. These data show a protective effect of Algerian propolis extract against doxorubicin-induced oxidative stress.
    Indian Journal of Nephrology 04/2011; 21(2):101-6. DOI:10.4103/0971-4065.82131
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