Article

Prevalence of legume sensitization in patients with naso-bronchial allergy.

Food Toxicology Division, Indian Institute of Toxicology Research, Lucknow, Uttar Pradesh, India.
Immunopharmacology and Immunotoxicology (impact factor: 1.83). 08/2008; 30(3):529-42. DOI:10.1080/08923970802135294 pp.529-42
Source: PubMed

ABSTRACT Prevalence of legume allergy and concomitant sensitization to different allergens was evaluated in patients of asthma and/ or rhinitis. Skin prick test with 27 common food allergens and 61 aeroallergens in 76 subjects was performed. Out of 76 subjects, 41 perceived allergenic problems with different food items as contributing factor. Only six patients showed prick test positivity compatible with their anamnesis. However, 35 subjects showed sensitization to different food items, out of which thirty were sensitive to legumes. The common foods in decreasing order of allergenicity were chickpea, followed by green gram, egg white, and bean fresh/red gram. Concomitant sensitization to different legume crops was found in patients.

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    Article: Kidney bean: a major sensitizer among legumes in asthma and rhinitis patients from India.
    Ramkrashan Kasera, Bhanu Pratap Singh, Sakuntala Lavasa, Komerla N Prasad, Ramesh C Sahoo, Anand B Singh
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    ABSTRACT: The prevalence of IgE mediated food allergies has increased over the last two decades. Food allergy has been reported to be fatal in highly sensitive individuals. Legumes are important food allergens but their prevalence may vary among different populations. The present study identifies sensitization to common legumes among Indian population, characterizes allergens of kidney bean and establishes its cross reactivity with other legumes. Patients (n = 355) with history of legume allergy were skin prick tested (SPT) with 10 legumes. Specific IgE (sIgE) and total IgE were estimated in sera by enzyme-linked immunosorbent assay. Characterization of kidney bean allergens and their cross reactivity was investigated by immunobiochemical methods. Identification of major allergens of kidney bean was carried out by mass spectrometry. Kidney bean exhibited sensitization in 78 (22.0%) patients followed by chickpea 65 (18.0%) and peanut 53 (15%). SPT positive patients depicted significantly elevated sIgE levels against different legumes (r = 0.85, p<0.0001). Sera from 30 kidney bean sensitive individuals exhibited basophil histamine release (16-54%) which significantly correlated with their SPT (r = 0.83, p<0.0001) and sIgE (r = 0.99, p<0.0001). Kidney bean showed eight major allergens of 58, 50, 45, 42, 40, 37, 34 and 18 kDa on immunoblot and required 67.3±2.51 ng of homologous protein for 50% IgE inhibition. Inhibition assays revealed extensive cross reactivity among kidney bean, peanut, black gram and pigeon pea. nLC-MS/MS analysis identified four allergens of kidney bean showing significant matches with known proteins namely lectin (phytohemagglutinin), phaseolin, alpha-amylase inhibitor precursor and group 3 late embryogenesis abundant protein. Among legumes, kidney bean followed by chick pea and peanut are the major allergic triggers in asthma and rhinitis patients in India. Kidney bean showed eight major allergens and cross reacted with other legumes. A combination of SPT, sIgE and histamine release assay is helpful in allergy diagnosis.
    PLoS ONE 01/2011; 6(11):e27193. · 4.09 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

27 common food allergens
 
76 subjects
 
asthma and/
 
common foods
 
concomitant sensitization
 
decreasing order
 
different food items
 
different legume crops
 
green gram
 
legume allergy
 
legumes
 
patients
 
prick test positivity compatible
 
sensitization
 
Skin prick test