Article

Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker.

Division of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 609-735, Republic of Korea.
Journal of Cancer Research and Clinical Oncology (impact factor: 2.56). 08/2008; 135(2):265-70. DOI:10.1007/s00432-008-0445-8 pp.265-70
Source: PubMed

ABSTRACT DYX1C1 has three alternatively spliced transcripts. Therefore, we expect that alternative transcripts of DYX1C1 are used as a biomarker to detect specific cancer.
RT-PCR analysis is conducted in order to detect expression of the DYX1C1 gene and the PCR products were analyzed using the Image J program to compare the expression levels of each transcript.
We found one of the transcripts was directly associated with an HERV-H LTR element that could be translated into protein sequence. Four new alternative transcripts were identified by RT-PCR analysis with various human tissue samples including 10 normal and adjacent tumor tissue sets. Semi-quantitative RT-PCR analysis showed the transcriptional activity of V3 and V2 was higher in tumor than in normal tissue samples, especially in the colorectal tissue samples.
Our results indicated that alternatively spliced transcript variants of the DYX1C1 gene could be used as cancer biomarkers to detect colorectal cancer.

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Keywords

10 normal
 
adjacent tumor tissue sets
 
biomarker
 
cancer biomarkers
 
colorectal tissue samples
 
DYX1C1 gene
 
expression levels
 
HERV-H LTR element
 
Image J program
 
normal tissue samples
 
PCR products
 
RT-PCR analysis
 
Semi-quantitative RT-PCR analysis
 
transcriptional activity
 
various human tissue samples