Sequence and phylogenetic analysis of a Chinese very virulent infectious bursal disease virus.
ABSTRACT The complete genome sequence of a Chinese very virulent infectious bursal disease virus (vvIBDV) strain, Harbin-1, was determined. Based on the sequence analysis, the molecular characteristics and potential virulence determinants and origin of vvIBDV strains were identified. Phylogenetic analysis indicated that a reassortment and/or recombination event may have occurred in the emergence of Chinese vvIBDV strains.
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ABSTRACT: Infectious bursal disease virus (IBDV) causes Gumboro disease, which is highly contagious and immunosuppressive in young chickens. A virulent form of IBDV reached South Africa in 1989 and to date there has been little molecular information available for this strain. In this study, the polyprotein coding region of the South African strain SA-KZN95 was sequenced and analysed along with 52 representative sequences of other serotype I and II strains. We explored the relative impact of recombination on phylogenetic reconstruction using a multidimensional scaling approach. Phylogenetic analyses consistently placed the South African isolate within the very virulent IBDV clade. Selection analyses were also conducted to identify evolutionarily relevant amino acid residues. Previously, 19 residues in the polyprotein were shown to be potentially diagnostic for the different IBDV pathotypes. This study identified an additional two unique residues in the polyprotein which may be used as genetic signatures in future viral identifications. Better strain identification would aid in the development and application of vaccines.Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases 11/2013; 21. DOI:10.1016/j.meegid.2013.11.017 · 3.26 Impact Factor
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ABSTRACT: The complete genome sequence of infectious bursal disease virus (IBDV) YS07 strain with low mortality isolated from Guangdong province of China in 2007 was reported in this study. The genome sequences and deduced amino acid (aa) sequences of YS07 were compared with that of previously reported IBDV strains. Phylogenetic analyses based on the deduced aa sequences of VP5, polyprotein, and VP1 protein revealed that YS07 bears its origin from European very virulent (vv) IBDV strains. Multiple aa sequences alignment revealed that YS07 contained a diversified genetic composition being characteristic to vv, variant, classical, and attenuated IBDV strains as well as its own unique ones. Our findings suggest that IBDV strains in China may gradually experience adaptive evolution due to immune pressure from intensive vaccination and demonstrate that the determination and analysis of full genome sequences is a valuable tool for studying the relationship between genetic composition and pathogenicity of IBDV strains.Virus Genes 07/2009; 39(2):246-8. DOI:10.1007/s11262-009-0379-5 · 1.84 Impact Factor
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ABSTRACT: A SYBR Green I based one-step real-time reverse transcriptase polymerase chain reaction was developed for the detection and differentiation of very virulent (vv) and classical strains of infectious bursal disease virus (IBDV). The assay showed high PCR efficiency >93% and high reproducibility with coefficient of variation less than 0.5%. When tested on characterized IBDV strains, the very virulent and classical-specific primers detected accurately only vvIBDV and classical IBDV strains, respectively. The diagnostic efficacy of the assay was also tested on 140 bursal samples from experimental infection and 37 bursal samples from cases suspected of IBD. The assay was able to detect IBDV from bursal samples collected at days 3 and 5 post-infection with the vvIBDV strain UPM94/273 and the classical IBDV strain D78. The assay was also able to detect bursal samples infected dually with D78 and UPM94/273. The melting temperature values of the amplification products from the classical and very virulent viral infection were statistically significant (P<0.05). The specificity of the assay for detecting IBDV from suspected cases was confirmed by sequence analysis of the VP2 gene. The assay showed high sensitivity since bursal samples which were negative for IBDV were confirmed by virus isolation and PCR amplification. Hence, the new assay offers an attractive method for rapid detection of strains of IBDV.Journal of virological methods 07/2009; 161(2):271-9. DOI:10.1016/j.jviromet.2009.06.023 · 1.88 Impact Factor