Article

Adenovirus-mediated gene expression imaging to directly detect sentinel lymph node metastasis of prostate cancer.

Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging and Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California 90095-1735, USA.
Nature medicine (impact factor: 27.14). 08/2008; 14(8):882-8. DOI:10.1038/nm.1727 pp.882-8
Source: PubMed

ABSTRACT The accurate assessment of nodal involvement in prostate cancer is crucial to planning treatment, yet there is a shortage of noninvasive imaging techniques capable of visualizing nodal lesions directly. This study demonstrates the feasibility of using recombinant human adenoviral vectors to detect nodal metastases in a human prostate cancer model. This was achieved by the prostate-restricted expression of optical and positron emission tomography (PET) imaging reporter genes by the viral vector coupled with the innate lymphotropic properties of adenovirus. We show that peritumoral administration of these vectors results in the direct detection of reporter gene expression in metastatic lesions within sentinel lymph nodes. Notably, this approach parallels the current lymphoscintigraphy method but enables the direct PET visualization of sentinel lymph node metastases, eliminating the need for invasive lymphadenectomy. These findings may lead to more effective diagnostic and therapeutic strategies for individuals with advanced-stage prostate cancer.

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Keywords

accurate assessment
 
advanced-stage prostate cancer
 
approach parallels
 
current lymphoscintigraphy method
 
direct detection
 
direct PET visualization
 
feasibility
 
human prostate cancer model
 
innate lymphotropic properties
 
invasive lymphadenectomy
 
noninvasive imaging techniques capable
 
optical
 
peritumoral administration
 
planning treatment
 
prostate cancer
 
prostate-restricted expression
 
sentinel lymph node metastases
 
sentinel lymph nodes
 
therapeutic strategies