Antioxidant Potential and Type II Diabetes-Related Enzyme Inhibition of Cassia obtusifolia L.: Effect of Indigenous Processing Methods
The methanolic extract of Cassia obtusifolia L. (Sicklepod) seed, an underutilized food legume from India, was analyzed for antioxidant and health relevant functionality. The total free phenolic content of the raw seeds was 13.33 ± 1.73 g catechin equivalent/100 g extract. The extract exhibited 1,292 mmol Fe[II] per milligram extract of ferric reducing/antioxidant power, 49.92% inhibition of ß-carotene degradation, 65.79% of scavenging activity against DPPH, and 50.78% of superoxide radicals. The in vitro starch digestion bioassay of the extract showed 79.80% of α-amylase and 81.04% of α-glucosidase enzyme inhibition characteristics. Sprouting + oil frying caused an apparent increase on the total free phenolic content with significant improvement on the antioxidant and free radical scavenging capacity of C. obtusifolia seeds, while soaking + cooking as well as open-pan roasting treatments show diminishing effects. Inhibition of α-amylase and α-glucosidase enzyme activity was 23.81% and 42.36%, respectively, following sprouting + oil-frying treatment. These enzyme inhibition values were similar to that of synthetic antidiabetic agent acarbose.
Available from: Yuan-yuan Lu
- "Semen Cassiae is the dry ripe seed of C. obtusifolia (Leguminosae). This herb is distributed mainly in China, Korea, India and the west tropical regions (Vadivel et al. 2012). The seeds are reported to have the effects of improving eyesight, alleviating constipation and lowering hypertension and hyperlipidemia (Hao et al. 2001). "
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ABSTRACT: Seeds of Cassia obtusifolia L. are known as homology of medicine and food material, which is a commonly consumed beverage in China. One new compound, 8-hydroxy-1,7-dimethoxy-3-methylanthracene-9,10-dione-2-O-β-d-glucoside (1), together with 11 known compounds, including seven anthraquinones (2-8), was isolated from the seeds. The 2D NMR data of compound 2 are reported for the first time. The structures of the compounds were established on the basis of 1D and 2D NMR, IR and HR-ESI-MS spectra. The cytotoxic activities of all the compounds against five cell lines (LO2, HCT-116, A549, HepG2 and SGC7901) were evaluated by using CCK8 methods. Compounds 1, 3 and 7 show moderate cytotoxicity towards HCT-116 cells compared with oxaliplatin.
Natural product research 04/2015; DOI:10.1080/14786419.2015.1032280 · 0.92 Impact Factor
Available from: Qifei Cong
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ABSTRACT: COB1B1S2 was isolated from an alkaline extract of Cassia obtusifolia seeds, and purified by anion-exchange and gel permeation chromatography. It contains arabinose, xylose, and glucuronic acid, in the molar ratio of 5:81:14, with an apparent molecular weight estimated to be 70.4kDa. Elucidated by using chemical and spectroscopic methods, COB1B1S2 was shown to have a backbone consisting of 1,4-linked β-d-Xylp, with one single-unit terminal α-d-GlcpA or α-l-Araf substituted at O-2 for nearly every five 1,4-linked Xylp. COB1B1S2 is structurally different from typical glucuronoxylans by its absence of methylation at O-4 of GlcA. The native COB1B1S2 showed no significant inhibition on the tube formation of human microvascular endothelial cells (HMEC) and on the growth of liver and colon cancer cells. On the contrary, COB1B1S2-Sul, prepared as the sulfated derivative of COB1B1S2, exhibited a significant inhibition on tube formation of HMEC in a dose-dependent manner, and on the growth of Bel7402 liver cancer cells. These results indicated that the introduction of sulfate groups significantly enhanced the biological activity of glucuronoxylan.
Carbohydrate Research 07/2014; 393. DOI:10.1016/j.carres.2014.04.016 · 1.93 Impact Factor
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ABSTRACT: Pulp and paper industry generates 30–180 m3 of wastewater per ton of manufactured pulp and 20–70 m3 of wastewater per ton of manufactured paper and paperboard. Coagulation process is widely applied as a pre-treatment or primary treatment to remove suspended solids from industrial effluent including pulp and paper mill effluent (PPME). Nevertheless, the excessive use of inorganic coagulants, such as alum, poses deleterious environmental impacts and risks to living organisms include low biodegradability, increase of metal content in discharged effluent and generation of toxic sludge. In view of this, the present study investigated the potential use of natural Cassia obtusifolia seed gum in treatment of raw and undiluted PPME through coagulation process. Recommended conditions (initial pH 5, 0.75 g/L dosage, 10 rpm and 10 min slow-mixing, and 1 min settling time) allowed C. obtusifolia gum removed high total suspended solids and chemical oxygen demand up to 86.9 and 36.2%, respectively. Findings from the present study showed that the coagulation efficiency using C. obtusifolia gum was comparable to alum. Also, characterizations showed that C. obtusifolia gum, alum and their flocs possessed distinctive features. The difference in peak occurrence from Fourier-transform infrared spectroscopy analysis indicated that the mechanism of floc formation using C. obtusifolia gum and alum differed. Besides, dissimilar thermal decomposition stages were observed for C. obtusifolia gum and alum through thermogravimetric analysis. Scanning electron microscope images showed that flocs formed using C. obtusifolia gum was highly fibrous-like and aggregate, whereas irregularly-shaped and aggregate for alum. In conclusion, C. obtusifolia gum could be served as a promising alternative to alum as a natural coagulant in treatment of PPME.
Industrial Crops and Products 11/2014; 61:317–324. DOI:10.1016/j.indcrop.2014.06.055 · 2.84 Impact Factor
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