Article

Preparation and characterization of guanidinated N-3-aminopropyl methacrylamide-N-2-hydroxypropyl methacrylamide copolymers as gene delivery carriers.

Biomaterials and Drug Delivery Laboratories, and School of Chemistry and Chemical Engineering, Southeast University, P.O. Box 211189, Nanjing, China.
Journal of Controlled Release (impact factor: 5.73). 11/2011; 152 Suppl 1:e168-70. DOI:10.1016/j.jconrel.2011.08.068
Source: PubMed

ABSTRACT N-3-guanidinopropyl methacrylamide-N-2-hydroxypropyl methacrylamide copolymers (GPMA-HPMA) synthesized via radical copolymerization of N-3-aminopropyl methacrylamide (APMA) and N-2-hydroxypropyl methacrylamide (HPMA) followed by guanidination were designed to develop novel non-viral gene carriers with cell membrane-penetrating properties. Synthesized GPMA-HPMA copolymers complex with pDNA completely at charge ratios no more than 1, and the positively charged complexes have diameters about 200nm. Furthermore, the guanidinated copolymers showed significantly decreased cytotoxicity, efficient transfection at low charge ratios with pDNA, and good nuclear localization ability.

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Keywords

APMA
 
cell membrane-penetrating properties
 
charge ratios
 
charged complexes
 
cytotoxicity
 
efficient transfection
 
good nuclear localization ability
 
guanidination
 
low charge ratios
 
N-3-guanidinopropyl methacrylamide-N-2-hydroxypropyl methacrylamide copolymers
 
novel non-viral gene carriers
 

Zhu Qin