Article

Assay to measure oxidized and reduced forms of CoQ by LC-MS/MS.

Seattle Children's Hospital Research Institute, Seattle, WA, USA.
Methods in molecular biology (Clifton, N.J.) 01/2012; 837:169-79. DOI:10.1007/978-1-61779-504-6_11 pp.169-79
Source: PubMed

ABSTRACT The redox status of mitochondrial coenzyme Q (CoQ) is an important marker for oxidative stress associated with several disorders such as Parkinson disease and Alzheimer disease. Altered redox status may be present in mitochondrial electron transport complex disorders. Intracellular CoQ levels reflect the functional status of the mitochondrial electron transport complex better than plasma levels. Here, we describe the method to determine the reduced and oxidized form of CoQ in white blood cells using LC-MS/MS.

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Keywords

Altered redox status
 
Alzheimer disease
 
CoQ
 
disorders
 
functional status
 
Intracellular CoQ levels
 
LC-MS/MS
 
mitochondrial coenzyme Q
 
mitochondrial electron transport complex
 
mitochondrial electron transport complex disorders
 
oxidized form
 
Parkinson disease
 
redox status
 
white blood cells