A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples

Biofunctional Nanomaterials Department, CICbiomaGUNE, Paseo Miramon 182, 20009 San Sebastian, Spain.
Chemical Communications (Impact Factor: 6.72). 12/2011; 48(11):1701-3. DOI: 10.1039/c2cc16537f
Source: PubMed

ABSTRACT A new surface-based MALDI-Tof-MS glycosyl hydrolase assay has been developed in which lipid-tagged oligosaccharides, representing defined fragments of major plant cell wall polysaccharides, are immobilized via hydrophobic interactions on an alkylthiol functionalised gold sample plate and employed in the functional screening of several purified enzymes, environmental samples and saliva.

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