Tea catechin epigallocatechin gallate inhibits Streptococcus mutans biofilm formation by suppressing gtf genes

Department of Pediatric Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA.
Archives of oral biology (Impact Factor: 1.74). 12/2011; 57(6):678-83. DOI: 10.1016/j.archoralbio.2011.10.021
Source: PubMed


The anti-cariogenic properties of tea have been suggested for decades. Tea polyphenols, especially epigallocatechin gallate (EGCG), have been shown to inhibit dental plaque accumulation, but the exact mechanisms are not clear at present. We hypothesise that EGCG suppresses gtf genes in Streptococcus mutans at the transcriptional level disrupting the initial attachment of S. mutans and thus the formation of mature biofilms.
In this study, the effect of EGCG on the sucrose-dependent initial attachment of S. mutans UA159 in a chemically defined medium was monitored over 4 h using a chamber slide model. The effects of EGCG on the aggregation and gtf B, C, D gene expression of S. mutans UA159 were also examined.
It was found that EGCG (7.8-31.25 μg/ml) exhibited dose-dependent inhibition of the initial attachment of S. mutans UA159. EGCG did not induce cellular aggregation of S. mutans UA159 at concentrations less than 78.125 μg/ml. Analysis of data obtained from real-time PCR showed that EGCG at sub-MIC level (15.6 μg/ml) significantly suppressed the gtf B, C, D genes of S. mutans UA159 compared with the non-treated control (p < 0.05).
These findings suggest that EGCG may represent a novel, natural anti-plaque agent that inhibits the specific genes associated with bacterial biofilm formation without necessarily affecting the growth of oral bacteria.

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Available from: Xin Xu, Aug 08, 2015
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    • "gtfBgtfCgtfD pH [13] gtfBgtfC pH [14] Koo [15] gtfBgtfC gtfDgtfBgtfC gtfBgtfC gtfD gtfBgtfC [] [1] Cummins D. Dentin hypersensitivity: from diagnosis to a breakthrough therapy for everyday sensitivity relief[J]. J "
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    ABSTRACT: To evaluate the effect of an arginine-containing antihypersensitivity polishing paste on Streptococcus mutans (S. mutans) adhesion to treated dentin. Dentin discs were treated with acid to expose dentin tubules, and then polished with either pumice or a polishing paste containing arginine. The surface roughness of the treated dentin was measured. The effects of dentin treatment on S. mutans adhesion and glucosyltransferase (GTFs) gene expression were also evaluated. The surface roughness decreased after polishing with both pumice and arginine-containing polishing paste. Moreover, the polishing paste affected gtfB and gtfC expressions. The arginine-containing polishing paste affects S. mutans adhesion, as well as gtfB and gtfC expressions. The polishing paste may be used to prevent caries in exposed dentin areas.
    Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology 10/2013; 31(5):453-6.
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    • "Interestingly, a study by Xu, et al.34 (2011) demonstrated that EGCg inhibited biofilm formation of S. mutans which could be attributed to the interactions of EGCg with glucosyltransferase enzymes, thus disrupting the formation and integrity of the oral biofilm. More recently, Xu, et al.35 (2012) also demonstrated that EGCg suppresses the gtfB, gtfC, gtfD genes associated with extracellular polysaccharide formation of S. mutans. The potential anticariogenic activity of EGCg in clinical service clearly requires more research, since EGCg has been shown to interact with salivary proteins. "
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    ABSTRACT: Objective: To test the inhibitory growth activity of green tea catechin incorporated into dental resins compared to resins containing the broad-spectrum antimicrobial compound chlorhexidine against Streptococcus mutans in vitro. Material and Methods: The minimum inhibitory concentrations (MICs) of epigallocatechin-gallate (EGCg) and chlorhexidine (CHX) were determined according to the microdilution method. Resin discs (5 mm x 3 mm) were prepared from Bis-GMA/TEGDMA (R1) and Bis-GMA/CH3Bis-GMA (R2) comonomers (n=9) containing: a) no drug, b) EGCg, c) CHX. Two concentrations of each drug (0.5x MIC and 1x MIC) were incorporated into the resin discs. Samples were individually immersed in a bacterial culture and incubated for 24 h at 37º C under constant agitation. Cell viability was assessed by counting the number of colonies on replica agar plates. Statistical analysis was performed using one-way ANOVA, Tukey and Student t-tests (α=0.05). Results: Both resins containing EGCg and CHX showed a significant inhibition of bacterial growth at both concentrations tested (p<0.05). A significantly higher inhibition was observed in response to resins containing CHX at 0.5x MIC and 1x MIC, and EGCg at 1x MIC when compared to EGCg at 0.5x MIC. Also, EGCg at 0.5x MIC in R1 had a significantly higher growth inhibition than in R2. Conclusions: Both EGCg and CHX retained their antibacterial activity when incorporated into the resin matrix. EGCg at 1x MIC in R1 and R2 resins significantly reduced S. mutans survival at a level similar to CHX. The data generated from this study will provide advances in the field of bioactive dental materials with the potential of improving the lifespan of resin-based restorations.
    Journal of applied oral science: revista FOB 03/2013; 21(2). DOI:10.1590/1678-7757201302430 · 0.92 Impact Factor
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    ABSTRACT: The purpose of this study was to evaluate the antibacterial potential and physicochemical properties of a dental adhesive incorporated with epigallocatechin-3-gallate (EGCG) in different concentration over time. EGCG was incorporated at a ratio of 100, 200, and 300 μg/ml into a dental adhesive. The effects of the cured adhesives on the growth of Streptococcus mutans were determined by direct contact test immediately or one month later and by scanning electron microscopy (SEM), respectively. Microtensile bond strength (μTBS) test was used to test the mechanical property of the adhesives immediately or six months later. The degree of conversion (DC) of the adhesives was evaluated by Fourier transform infrared spectroscopy (FTIR). Compared with negative control, the 200 μg/ml and 300 μg/ml EGCG-incorporated dental adhesive were found to exhibit inhibitory effect on the growth of S. mutans. The μTBS of the EGCG-incorporated dental adhesive was higher than the control. The DC of the adhesive system was not affected by the addition of EGCG. 200 μg/ml EGCG incorporated dental adhesives could accomplish therapeutic goals that play in antimicrobial function whilst keeping the durability of resin-dentine bond.
    Journal of dentistry 03/2012; 40(6):485-92. DOI:10.1016/j.jdent.2012.02.013 · 2.75 Impact Factor
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