ENAC1, a NAC Transcription Factor, is an Early and Transient Response Regulator Induced by Abiotic Stress in Rice (Oryza sativa L.)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
Molecular Biotechnology (Impact Factor: 1.88). 12/2011; 52(2):101-10. DOI: 10.1007/s12033-011-9477-4
Source: PubMed


The plant-specific NAC (NAM, ATAF, and CUC)-domain proteins play important roles in plant development and stress responses. In this research, a full-length cDNA named ENAC1 (early NAC-domain protein induced by abiotic stress 1) was isolated from rice. ENAC1 possess one NAC domain in the N-terminus. Comparative time-course expression analysis indicated that ENAC1 expression, similar with OsDREB1A, was induced very quickly by various abiotic stresses including salt, drought, cold, and exogenous abscisic acid. However, the induction of ENAC1 by abiotic stress was transient and lasted up to 3 h, whereas that of OsDREB1A maintained longer. The promoter sequence of ENAC1 harbors several cis-elements including ABA response elements, but the well-known dehydration responsive element/C-repeat element is absent. The ENAC1-GFP (green fluorescent protein) fusion protein was localized in the nucleus of rice protoplast cell. Yeast hybrid assays revealed that ENAC1 was a transcription activator and bound to NAC recognition sequence (NACRS). Co-expression analysis suggested that ENAC1 co-expressed with a number of stress-related genes. Taken together, ENAC1 may be an early transcription activator of stress responses and function in the regulation of NACRS-mediated gene expression under abiotic stress.

28 Reads
  • Source
    • "In contrast, Liu et al. (2014) had previously identified 11 Cd stress-responsive NAC genes from the ramie subjected to only one week of Cd stress treatment. Generally, the expression of regulatory genes, such as NAC TFs, is induced in the early stages of environment stress, and thereafter, these genes activating/inhibiting the expression of their downstream genes in the pathway, and finally resulting in the plant adaptation to the environment stress (Sun et al., 2012). In other words, a stress treatment of 20 days might lead the ramie plants into the late stages of Cd stress response when no NAC genes showed differential expression. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Cadmium (Cd) contamination in agricultural soils has become a major environmental problem in China. Ramie, a fiber crop, has frequently been proposed for use as a phytoremediation crop for the restoration of Cd-contaminated farmlands. However, high levels of Cd can greatly inhibit stem growth in ramie, which reduces its economic value as a crop. To understand the potential mechanisms behind this phenomenon, the ramie genes involved in the Cd stress response were identified using Illumina pair-end sequencing on two Cd-stressed plants (CdS1 and CdS2) and two control plants (CO1 and CO2). Approximately 48.7, 51.6, 41.2, and 47.1 million clean sequence reads were generated from the libraries of CO1, CO2, CdS1, and CdS2, respectively, and de novo assembled to yield 56,932 non-redundant unigenes. A total of 26,686 (46.9%) genes were annotated for their function. Comparison of gene expression levels in CO and CdS ramie revealed 155 differentially expressed genes (DEGs) between treatment and control conditions. Sixteen DEGs were further analyzed for expression differences by using real-time quantitative PCR (qRT-PCR). Among these 16 DEGs, 2 genes encoding GA2-oxidase (a major enzyme for deactivating bioactive gibberellins [GAs]) showed markedly up-regulated expression in Cd stressed ramie. This might be responsible for the growth inhibition of Cd-stressed ramie. Pathway enrichment analysis revealed that the cutin, suberine and wax biosynthesis pathway was markedly enriched by DEGs. The discovery of these Cd stress-responsive genes and pathways will be helpful in further understanding the mechanism of Cd-stress response and improving Cd stress tolerance in ramie. Copyright © 2014. Published by Elsevier B.V.
    Gene 12/2014; 558(1). DOI:10.1016/j.gene.2014.12.057 · 2.14 Impact Factor
  • Source
    • "japonica) cultivar Jiucaiqing, the rice ABI5-Like1 (ABL1) deficiency mutant, abl1, and its wild type Zhonghua 11 (ZH11, japonica) were sterilized in 0.1% HgCl2 and germinated at 30°C. The seedlings were cultured with Yoshida's culture solution in growth chamber as previously described [20]. The 2-week-old seedlings were used for subsequent stress treatments. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Homeodomain-leucine zipper type I (HD-Zip I) proteins are involved in the regulation of plant development and response to environmental stresses. In this study, OsSLI1 (Oryza sativa stress largely induced 1), encoding a member of the HD-Zip I subfamily, was isolated from rice. The expression of OsSLI1 was dramatically induced by multiple abiotic stresses and exogenous abscisic acid (ABA). In silico sequence analysis discovered several cis-acting elements including multiple ABREs (ABA-responsive element binding factors) in the upstream promoter region of OsSLI1. The OsSLI1-GFP fusion protein was localized in the nucleus of rice protoplast cells and the transcriptional activity of OsSLI1 was confirmed by the yeast hybrid system. Further, it was found that OsSLI1 expression was enhanced in an ABI5-Like1 (ABL1) deficiency rice mutant abl1 under stress conditions, suggesting that ABL1 probably negatively regulates OsSLI1 gene expression. Moreover, it was found that OsSLI1 was regulated in panicle development. Taken together, OsSLI1 may be a transcriptional activator regulating stress-responsive gene expression and panicle development in rice.
    06/2014; 2014:809353. DOI:10.1155/2014/809353
  • Source
    • "Plants adjust to these stresses through various biochemical and physiological processes, including the accumulation of sugars, proline, and glycine betaine and increasing the expression levels of many genes such as protein kinases (Shinozaki and Yamaguchi-Shinozaki 2000). Protein kinases include mitogen-activated protein (MAP) kinases, calcium-dependent protein kinases (CDPKs), receptorlike kinases, histidine kinases, and serine/threonine protein kinases (Rabbani et al. 2003; Zhou et al. 2007; Sun et al. 2012). Cyclin-dependent kinases (CDKs) are a large family of serine/threonine protein kinases that play important roles in cell proliferation (Doonan and Kitsios 2009). "
    [Show abstract] [Hide abstract]
    ABSTRACT: In our previous research, we showed that the cyclin-dependent kinase regulatory subunit (CKS2) in maize (Zea mays L.) was induced by water deficit and cold stress. To elucidate its expression patterns under adversity, we isolated and characterized its promoter (PZmCKS2). A series of PZmCKS2-deletion derivatives, P0–P3, from the translation start code (−1,455, −999, −367, and −3 bp) was fused to the β-glucuronidase (GUS) reporter gene, and each deletion construct was analyzed by Agrobacterium-mediated steady transformation into Arabidopsis. Leaves were then subjected to dehydration, cold, abscisic acid (ABA), salicylic acid (SA), and methyl jasmonic acid (MeJA). Sequence analysis showed that several stress-related cis-acting elements (MBS, CE3, TGA element, and ABRE) were located within the promoter. Deletion analysis of the promoter, PZmCKS2, suggested that the −999 bp promoter region was required for the highest basal expression of GUS, and the −367 bp sequence was the minimal promoter for ZmCKS2 activation by low temperature, ABA, and MeJA. The cis-acting element ABRE was necessary for promoter activation by exogenous ABA.
    Acta Physiologiae Plantarum 05/2014; 36(7). DOI:10.1007/s11738-014-1563-3 · 1.58 Impact Factor
Show more