Article

Capillary electrophoresis.

University of Minnesota, Department of Chemistry, 207 Pleasant Street South East, Minneapolis, Minnesota 55455, United States.
Analytical Chemistry (Impact Factor: 5.83). 12/2011; 84(2):577-96. DOI: 10.1021/ac203205a
Source: PubMed

ABSTRACT Capillary Electrophoresis (CE) is a field that continues to grow. All areas of CE including theory, separation modes, instrumentation and applications remain highly active areas of research. This review includes a cross section of references from all areas of the field published in the two year period between Jan. 2010 and Nov. 2011. Web of Science reports over 4,000 articles, including 396 reviews, published with CE in the title, abstract or key words during this time period. Of these we have chosen 218 papers. We have attempted to choose papers that showcase some of the newest and most exciting developments in the field. It should be noted that papers describing electrophoresis in microfabricated devices were excluded since another review in this issue exclusively covers this topic.

0 Bookmarks
 · 
133 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Thirteen mono-N-acyl derivatives of 2,6-diaminopimelic acid (DAP) - new potential inhibitors of the DapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE; EC 3.5.1.18) were analyzed and characterized by IR and NMR spectroscopies and two capillary electromigration methods - zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC). Structural features of DAP derivatives were characterized by IR and NMR spectroscopies whereas CZE and MEKC were applied to evaluate their purity and to investigate their electromigration properties. Effective electrophoretic mobilities of these compounds were determined by CZE in acidic and alkaline background electrolytes (BGEs) and by MEKC in acidic and alkaline BGEs containing a pseudostationary phase of anionic detergent sodium dodecylsulfate (SDS) or cationic detergent cetyltrimethylammonium bromide. The best separation of DAP derivatives, including diastereomers of some of them, was achieved by MEKC in an acidic BGE (500 mM acetic acid, pH 2.54, 60 mM SDS). All DAP derivatives were examined for their ability to inhibit catalytic activity of DapE from Haemophilus influenzae and ArgE from Escherichia coli. None of these DAP derivatives worked as an effective inhibitor of HiDapE but one derivative, N-fumaryl, Me-ester-DAP, was found to be moderate inhibitor of EcArgE, thus providing a promising lead structure for further studies on ArgE inhibitors.
    Analytical Biochemistry 09/2014; · 2.31 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A graphene oxide coated capillary was fabricated by using 3-aminopropyltriethoxysilane as the cross-linking agent. It was used for the separation and detection of three endocrine-disrupting chemicals, including bisphenol A, 4-nonylphenol and 4-octylphenol by capillary electrochromatography. Due to the hydrophobicity, hydrogen bonding, and π–π interaction between graphene oxide and the analytes, the three analytes could be well separated in pH = 11.0, 20 mmol/L Na2B4O7-NaOH/methanol mobile phase (50:50, v/v) within 950 s. After preconcentration, the detection limits were 6.7×10−10, 3.3×10−9, and 6.7×10−10 mol/L (S/N = 3) for bisphenol A, nonylphenol, and octylphenol, respectively. The developed method was successfully applied to the determination of the above analytes in water samples. The satisfactory result demonstrated that the graphene oxide coated capillary used in capillary electrochromatography with amperometric detection was convenient to prepare, highly stable and had good reproducibility.This article is protected by copyright. All rights reserved
    Journal of Separation Science 04/2014; · 2.59 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Because of their high toxicity, international regulatory institutions recommend monitoring specific polycyclic aromatic hydrocarbons (PAHs) in environmental and food samples. A fast, selective and sensitive method is therefore required for their quantitation in such complex samples. This article deals with the optimization, based on an experimental design strategy, of a cyclodextrin (CD) modified capillary zone electrophoresis separation method for the simultaneous separation of 19 PAHs listed as priority pollutants. First, using a central composite design, the normalized peak-start and peak-end times were modelled as functions of the factors that most affect PAH electrophoretic behavior: the concentrations of the anionic sulfobutylether-β-CD and neutral methyl-β-CD, and the percentage of MeOH in the background electrolyte. Then, to circumvent computational difficulties resulting from the changes in migration order likely to occur while varying experimental conditions, an original approach based on the systematic evaluation of the time intervals between all the possible pairs of peaks was used. Finally, a desirability analysis based on the smallest time interval between two consecutive peaks and on the overall analysis time, allowed us to achieve, for the first time in CE, full resolution of all 19 PAHs in less than 18min. Using this optimized capillary electrophoresis method, a vegetable oil was successfully analyzed, proving its suitability for real complex sample analysis.
    Analytica chimica acta 04/2014; 820:195-204. · 4.31 Impact Factor

Preview

Download
1 Download
Available from