Capillary electrophoresis.

University of Minnesota, Department of Chemistry, 207 Pleasant Street South East, Minneapolis, Minnesota 55455, United States.
Analytical Chemistry (Impact Factor: 5.83). 12/2011; 84(2):577-96. DOI: 10.1021/ac203205a
Source: PubMed

ABSTRACT Capillary Electrophoresis (CE) is a field that continues to grow. All areas of CE including theory, separation modes, instrumentation and applications remain highly active areas of research. This review includes a cross section of references from all areas of the field published in the two year period between Jan. 2010 and Nov. 2011. Web of Science reports over 4,000 articles, including 396 reviews, published with CE in the title, abstract or key words during this time period. Of these we have chosen 218 papers. We have attempted to choose papers that showcase some of the newest and most exciting developments in the field. It should be noted that papers describing electrophoresis in microfabricated devices were excluded since another review in this issue exclusively covers this topic.

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    ABSTRACT: Thirteen mono-N-acyl derivatives of 2,6-diaminopimelic acid (DAP) - new potential inhibitors of the DapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE; EC were analyzed and characterized by IR and NMR spectroscopies and two capillary electromigration methods - zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC). Structural features of DAP derivatives were characterized by IR and NMR spectroscopies whereas CZE and MEKC were applied to evaluate their purity and to investigate their electromigration properties. Effective electrophoretic mobilities of these compounds were determined by CZE in acidic and alkaline background electrolytes (BGEs) and by MEKC in acidic and alkaline BGEs containing a pseudostationary phase of anionic detergent sodium dodecylsulfate (SDS) or cationic detergent cetyltrimethylammonium bromide. The best separation of DAP derivatives, including diastereomers of some of them, was achieved by MEKC in an acidic BGE (500 mM acetic acid, pH 2.54, 60 mM SDS). All DAP derivatives were examined for their ability to inhibit catalytic activity of DapE from Haemophilus influenzae and ArgE from Escherichia coli. None of these DAP derivatives worked as an effective inhibitor of HiDapE but one derivative, N-fumaryl, Me-ester-DAP, was found to be moderate inhibitor of EcArgE, thus providing a promising lead structure for further studies on ArgE inhibitors.
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    ABSTRACT: A new single-isomer cationic cyclodextrin, mono-6(A)-[3-(3-methoxypropyl)imidazol-1-ium]-6(A)-β-cyclodextrin chloride, has been synthesized and successfully used for the chiral separation of dansyl amino acids in capillary electrophoresis. With methoxy functionality, the new cationic cyclodextrin exhibits significantly improved enantioselectivities. Excellent enantioseparations for amino acids are obtained in chiral selector concentration range between 2.5mM to 15mM at pH 6.0. Chiral resolution as high as 7.3 was achieved for Dns-Aca with 5mM chiral selector. Comparison study and theoretical calculation with Wren's model attribute the enhanced enantioseparation to the stronger inclusion complexation between amino acids and cyclodextrin. The binding constants for dansyl amino acids and the cationic cyclodextrins are calculated to be 173-253M(-1), while the optimum cyclodextrin concentrations were estimated to be 4.1-7.6mM.
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    ABSTRACT: A fraction collector based on a drop-on-demand ink-jet printer was developed to interface capillary zone electrophoresis with a 96 well microtiter plate. We first evaluated the performance of the collector by using capillary zone electrophoresis to analyze a 1mM solution of tetramethylrhodamine; a fluorescent microtiter plate reader was then used to detect the analyte and characterize fraction carryover between wells. Relative standard deviation in peak height was 20% and the relative standard deviation in migration time was 1%. The mean and standard deviation of the tetramethylrhodamine peak width was 5±1s and likely limited by the 4-s period between droplet deposition. We next injected a complex mixture of DNA fragments and used real-time PCR to quantify the product in a CE-SELEX experiment. The reconstructed electrophoretic peak was 27s in duration. Finally, we repeated the experiment in the presence of a 30-µM thrombin solution under CE-SELEX conditions; fractions were collected and next-generation sequencing was used to characterize the DNA binders. Over 25,000 sequences were identified with close matches to known thrombin binding aptamers.
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