Article

Evaluation of antiulcerogenic activity of aqueous extract of Brassica oleracea var. capitata (cabbage) on Wistar rat gastric ulceration.

Departamento de Biologia Geral, Universidade Federal de Viçosa, Brazil.
Arquivos de gastroenterologia 12/2011; 48(4):276-82. DOI: 10.1590/S0004-28032011000400011
Source: PubMed

ABSTRACT Abstract
CONTEXT:
The cabbage (Brassica oleraceae var. capitata) is an herbaceous and leafy plant which belongs to the Brassicaceae family, native to coastal southern and Western Europe. Used in cooking for its nutritional value also has known anti-inflammatory activity. OBJECTIVE We studied the antiulcerogenic activity of aqueous extract of Brassica oleracea var. capitata (AEB) in order to validate ethnobotanical claims regarding the plant use in the gastric disorders.
METHOD:
Acute gastric ulcers were induced in rats by the oral administration of acetylsalicylic acid. The gastroprotective potential of the AEB (0.250, 0.500 and 1.000 mg.kg-1/body weight) was compared with omeprazole (20 mg.kg-1/body weight).
RESULTS:
The stomach analysis indicated that treatment with AEB inhibited the gastric damage. The gastroprotective activity as evidenced by its significant inhibition in the formation of ulcers induced by chemical agent with a maximum of 99.44% curation (250 mg.kg-1 body weight) in acetylsalicylic acid-induced ulcers.
CONCLUSIONS:
The AEB demonstrated good antiulcerogenic activities which justify the inclusion of this plant in the management of gastric disorders. Further experiments are underway to determine which antiulcer mechanisms involved in gastroprotection.

2 Followers
 · 
523 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Toll-like receptors (TLRs) play a key role in linking pathogen recognition with the induction of innate immunity. They have been implicated in the pathogenesis of chronic inflammatory diseases, representing potential targets for prevention/treatment. Vegetable-rich diets are associated with the reduced risk of several inflammatory disorders. In the present study, based on an extensive screening of vegetable extracts for the TLR-inhibiting activity in the HEK293 cells co-expressing TLR together with the NF-κB reporter gene, we found the cabbage and onion extracts as the richest sources of a TLR signaling inhibitor. To identify the active substances, we performed the activity-guiding separation of the principal inhibitors and identified 3-methylsulfinylpropyl isothiocyanate (iberin) from the cabbage and quercetin and quercetin-4'-O-β-glucoside from the onion, among which iberin showed the most potent inhibitory effect. It was revealed that iberin specifically acted on the dimerization step of TLRs in the TLR signaling pathway. To gain an insight into the inhibitory mechanism of the TLR dimerization, we developed a novel probe, combining an isothiocyanate-reactive group and an alkyne functionality, for click chemistry and detected the probe bound to the TLRs in living cells, suggesting that iberin disrupts dimerization of the TLRs via covalent binding. Furthermore, we designed a variety of iberin analogues and found that the inhibition potency was influenced by the oxidation state of the sulfur. Modeling studies of the iberin analogues showed that the oxidation state of sulfur might influence the global shape of the isothiocyanates. These findings establish the TLR dimerization step as a target of food-derived anti-inflammatory compounds.
    Journal of Biological Chemistry 10/2014; DOI:10.1074/jbc.M114.585901 · 4.60 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: INTRODUCTION Today, herbal remedies are back into prominence because the efficacy of conventional medicines such as antibiotics, which once had near-universal effectiveness against serious infections, is on the wane. Ayurveda-based drug discovery uses 'reverse pharmacology', in which drug candidates are first identified based on large scale use in the population, then validated in clinical trials. [1] With growing fondness to natural medicine, recent research is focused on tackling free radical involvement with antioxidant phytochemicals. Antioxidants combat oxidative stress by neutralizing excess free radicals and stopping them from starting the chain reactions that lead to Parkinsonism disease. [2] While some beneficial phytochemicals might function solely as antioxidants, it is becoming increasingly obvious that there could be other potential mechanisms by which these phytochemicals render protection. Several phytochemicals have been shown to protect neurons against injury and disease by stimulating the production of antioxidant enzymes, neurotrophic factors, protein chaperons and other proteins that help to withstand oxidative stress. [3] Neurodegenerative disorders remain an important source of morbidity and suffering for the humankind. Neurodegenerative diseases have long been defined by the properties of the neuropathological lesions observed in the brain. The role of free-radical mediated oxidative injury in acute insults to the nervous system including stroke or trauma, as well as in chronic neurodegenerative disorders, is being just recognized. [4] Approximately 1% of the population over the age of 65 and 4–5% of the population by the age of 85 suffer from the most common form of motor system degeneration and the second most common neurodegenerative disorder, Parkinson's disease. [5] Brassica Oleracea belongs to the family Brassicaceae is a leafy green vegetable commonly called as cabbage. [6] The plant was reported to contain different phytoconstituents like Phenolic compounds such as quercetin 3-O-sophoroside-7-O-glucoside, 3-p-coumaroylquinic acid, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-(caffeoyl)-sophoroside-7-O glucoside, sinapoylglucoside glucoside acid, kaempferol 3-O-(sinapoyl)-sophoroside 7-Oglucoside. [7] Organic acids such as aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric acids has been identified and quantified from Brassica oleracea. [8] Studies reveal that it is rich in flavonoids and is having high antioxidant property. [9] It is reported to have analgesic, anti-inflammatory, [10] anticarcinogenic, [11] antiulcer, [12] antidiabetic [13] activities. In the present study, an attempt was made to evaluate the antioxidant and antiparkinsonian activities of Brassica oleracea. MATERIALS AND METHODS Collection and Authentication of Plant Material Fresh cabbage is collected from local cultivating areas in and around of Anantapuramu district of Andhra Pradesh, India and it is identified and authenticated by Dr. J. Ravindra Reddy, Professor and Vice Principal, Raghavendra Institute of Pharmaceutical Education and Research, Anantapur and the voucher specimen (01/13) was preserved in the department of pharmacology, RIPER, for further reference. The collected plant material was chopped into small pieces and left to dry at room temperature. The shade dried cabbage pieces were made into powder mechanically.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Recent attraction and follow-up research on the genomic and biochemical studies of Brassica oleracea reveal the importance of BoSMT genes that trigger the cascade of responses leading to Selenium (Se) accumulation in model plants. On the other hand bioinformatics plays an essential role in today’s plant science. As the amount of data grows exponentially, there is a parallel growth in the demand for tools and methods in data management, visualization, integration, analysis, modeling, and prediction. Structure prediction is used both for assessing the quality of the newly determined structure and predicting the structure of proteins whose (BoSMT) sequences are newly determined. The necessary condition for successful homology modelling is to have sufficient similarity between the protein sequences. Here we construct the BoSMT enzyme protein structure based on the Thermotoga maritima (Tm-HMT) by using MODELER program. From Ramachandran plot analysis protein residues falling into the most favoured regions were determined (83.3%). The predicted molecular 3D model further verified with PROCHECK, VADAR online server to confirm the geometry and stereo-chemical parameters of molecular architecture. Successfully modelled, verified and the most reliable structure of BoSMT was used for deposition in PMDB (Protein Model Database) database accession No. PM0078717. The multiple sequence alignment of BoSMT with related plants showed high sequence homology (87% BoHMT, 72% Malus domestica, 70% Astragalus chrysochlorus, 69% A. racemicus and A. drummondii, 68% Camella sinesis, 65% Arabidopsis thaliana (AtHMT2), 53% Sorghum, 38% Zea mays). Moreover active binding site and functional implication were discussed for BoSMT enzyme.
    Brassicacease Characterization, Functional Genomic and Health Benefits, Biomedical edited by Minglin Lang, 04/2013: chapter Three-dimensional molecular structure prediction of selenocysenin methyltransferase (BoSMT) from Brassica oleracea.: pages 149-169; Nova Biomedical Science Publisher Inc.., ISBN: 978-1-62808-869-4

Full-text

Download
91 Downloads
Available from
May 21, 2014