Localization of acidic phospholipid cardiolipin and DnaA in mycobacteria

Biomedical Research, The University of Texas Health Science Center, 11937 US Hwy @ 271, Tyler, TX 75708-3154, United States.
Tuberculosis (Edinburgh, Scotland) (Impact Factor: 2.71). 12/2011; 91 Suppl 1:S150-5. DOI: 10.1016/j.tube.2011.10.025
Source: PubMed


Acidic phospholipids such as cardiolipin (CL) have been shown to modulate Mycobacterium tuberculosis (Mtb) DnaA interactions with ATP. In the present study, using nonyl acridine orange fluorescent dye we localized CL-enriched regions to midcell septa and poles of actively dividing cells. We also found that CL-enriched regions were not visualized in cells defective for septa formation as a consequence of altered FtsZ levels. Using Mtb cultures synchronized for DNA replication we show that CL localization could be used as a marker for cell division and cell cycle progression. Finally, we show that the localization pattern of the DnaA-green fluorescent fusion protein is similar to CL. Our results suggest that DnaA colocalizes with CL during cell cycle progression.

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Available from: Malini Rajagopalan, Dec 19, 2014
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    • "In contrast, in mycobacteria, poles are very active loci where lateral PG synthesis [22] and most likely free MA synthesis [52], occur during bacterial elongation. In addition, poles are also involved in protein export [53], DNA transfer [32], chromosomal partitioning and DNA replication [54], [55] as well as phosphorylation by Ser/Thr protein kinases [56]. Mycobacterial poles are thus certainly not suitable for detrimental protein accumulation. "
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