Effects of the interaction of TiO2 nanoparticles with bisphenol A on their physicochemical properties and in vitro toxicity
ABSTRACT In this paper we evaluated the effects of the interaction of TiO(2) nanoparticles (nano-TiO(2)) with bisphenol A (BPA) on their physicochemical properties and in vitro toxicity in human embryo L-02 hepatocytes. Different concentrations of BPA (0, 0.1, 1, 10 μmol/L) and nano-TiO(2) (0, 0.1, 1, 10mg/L) were mixed to analyze the size distribution, zeta potential, adsorption capacity and uptake of nano-TiO(2), and the toxicity of nano-TiO(2) and BPA in L-02 cells. The addition of BPA to nano-TiO(2) dispersions increased the aggregation level and zeta potential of nano-TiO(2) in all media. Nano-TiO(2) had a similar adsorption capacity in different media, although a higher aggregation level was observed in cell culture medium. Nano-TiO(2), with or without BPA, could enter L-02 cells after 24h exposure. Nano-TiO(2) alone did not induce significant DNA and chromosome damage, but the mixture of nano-TiO(2) and BPA increased toxicity via increasing oxidative stress, DNA double strand breaks and micronuclei formation. The aggregated nano-TiO(2) can enrich BPA effectively. The BPA-bound nano-TiO(2) are proven to be uptaken into nuclei of exposed cells, which may increase intracellular BPA and nano-TiO(2) levels and thus lead to synergistic toxicity. However only small synergic effects were observed at the concentrations of BPA and nano-TiO(2) used in this study.
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- "These results show that such NPs have the potential to induce cytotoxicity and genotoxicity in cultured WISH cells . In another study, TiO2NPs alone did not induce significant DNA and chromosome damage in human embryo L-02 hepatocytes, but a mixture of TiO2NPs and bisphenol A increased toxicity by increasing oxidative stress, DNA double-strand breaks, and micronuclei formation . In one study Chinese hamster ovary cells appeared to adapt to chronic exposure to TiO2NPs and to detoxify excess ROS, possibly through the upregulation of SOD and by reducing particle uptake . "
ABSTRACT: The use of nanoparticles (NPs) in industry is increasing, bringing with it a number of adverse health effects on workers. Like other chemical carcinogens, NPs can cause cancer via oxidative DNA damage. Of all the molecules vulnerable to oxidative modification by NPs, DNA has received the greatest attention, and biomarkers of exposure and effect are nearing validation. This review concentrates on studies published between 2000 and 2012 that attempted to detect oxidative DNA damage in humans, laboratory animals, and cell lines. It is important to review these studies to improve the current understanding of the oxidative DNA damage caused by NP exposure in the workplace. In addition to examining studies on oxidative damage, this review briefly describes NPs, giving some examples of their adverse effects, and reviews occupational exposure assessments and approaches to minimizing exposure (e.g., personal protective equipment and engineering controls such as fume hoods). Current recommendations to minimize exposure are largely based on common sense, analogy to ultrafine material toxicity, and general health and safety recommendations.Safety and Health at Work 12/2013; 4(4):177-186. DOI:10.1016/j.shaw.2013.07.006
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ABSTRACT: Due to their chemical stability and nonallergic, nonirritant, and ultraviolet protective properties, titanium dioxide (TiO2) nanoparticles (NPs) have been widely used in industries such as electronics, optics, and material sciences, as well as architecture, medicine, and pharmacology. However, increasing concerns have been raised in regards to its ecotoxicity and toxicity on the aquatic environment as well as to humans. Although insights have been gained into the effects of TiO2 NPs on susceptible biological systems, there is still much ground to be covered, particularly in respect of our knowledge of the effects of the interaction of TiO2 NPs with other chemicals or physical factors. Studies suggest that interactions of TiO2 NPs with other chemicals or physical factors may result in an increase in toxicity or adverse effects. This review highlights recent progress in the study of the interactive effects of TiO2 NPs with other chemicals or physical factors.International Journal of Nanomedicine 07/2013; 8:2509-20. DOI:10.2147/IJN.S46919 · 4.38 Impact Factor
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ABSTRACT: A novel double-cylindrical-shell (DCS) photoreactor immobilized with monolayer TiO2-coated silica gel beads was developed and successfully applied for the degradation of Rhodamine B (RhB) and Methyl Orange (MO). Photocatalytic degradation of RhB using the developed photocatalytic reactor was confirmed by UV–VIS–NIR spectroscopy analysis and kinetic studies. The operational parameters including flow rate, initial concentration and repetitive operation for the degradation of RhB was investigated to optimize the developed photocatalytic reactor. Electrical energy per order (EEO) was used to calculate the electrical energy consumption for the degradation of RhB and MO using the developed photocatalytic reactor. The developed photocatalytic reactor, in comparison with reported slurry-suspension and thin-film photoreactors, showed higher efficiency, lower energy consumption and better repetitive operation performance for the degradation of RhB and MO. The TiO2-coated silica gel beads immobilized DCS photoreactor is a promising alternative for the decomposition of recalcitrant organic pollutants in wastewater.Separation and Purification Technology 02/2014; 123:130–138. DOI:10.1016/j.seppur.2013.12.029 · 3.09 Impact Factor