[Establishment of molecular beacon real-time PCR for detecting p53 gene single nucleotide polymorphism].
ABSTRACT To establish a method based on molecular beacon real-time PCR for detecting single nucleotide polymorphisms (SNP) in codon 72 of scar-related p53 gene.
Two fluorescence-labeled molecular beacon probes were synthesized targeting CCC/CGC SNP of p53 codon 72. The genomic DNA was extracted from the peripheral blood of 28 patients with keloid, and the CCC/CGC SNP of P53 gene codon 72 were assayed with molecular beacon real-time PCR. The results of SNP typing were compared with the results of reverse dot hybridization and confirmed by direct DNA sequencing.
The goodness of fit of this method was 100% in comparison with direct DNA sequencing, higher than that of reverse dot hybridization.
Molecular beacon real-time PCR is suitable for rapid clinical detection of SNPs in p53 gene.