Decoding cell lineage from acquired mutations using arbitrary deep sequencing

Department of Pathology, University of Washington School of Medicine, Seattle, Washington, USA.
Nature Methods (Impact Factor: 25.95). 11/2011; 9(1):78-80. DOI: 10.1038/nmeth.1781
Source: PubMed

ABSTRACT Because mutations are inevitable, the genome of each cell in a multicellular organism becomes unique and therefore encodes a record of its ancestry. Here we coupled arbitrary single primer PCR with next-generation DNA sequencing to catalog mutations and deconvolve the phylogeny of cultured mouse cells. This study helps pave the way toward construction of retrospective cell-fate maps based on mutations accumulating in genomes of somatic cells.

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