Circulating tissue factor positive microparticles in patients with acute recurrent deep venous thrombosis

Department of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Thrombosis Research (Impact Factor: 2.43). 11/2011; 130(2):253-8. DOI: 10.1016/j.thromres.2011.10.014
Source: PubMed

ABSTRACT Circulating tissue factor positive microparticles (MPTF) were reported in a wide range of diseases with thrombotic tendency. Though D-dimer assay had a high negative predictive value for deep venous thrombosis (DVT) recurrence, there are currently no reliable positive predictors for recurrent DVT. We therefore quantified MPTF in patients with acute recurrent DVT to determine whether MPTF levels could be used to predict recurrent DVT.
Microparticles (MPs) were isolated from plasma of initial DVT patients (n=25), recurrent DVT patients (n=25) and sex- and age-matched healthy individuals (n=25), stained with annexin V, cell-specific monoclonal antibodies (MoAbs) and a MoAb directed against tissue factor (TF), and analyzed by flow cytometry. We also determined the plasma procoagulant activity with a Human TF Chromogenic Activity Assay Kit.
We found total MPTF to be elevated in recurrent DVT patients versus normal individuals (P=0.001). The number of monocyte-derived MPTF in both initial and recurrent DVT was higher than in normal individuals (P<0.01, respectively). The platelet and endothelial cell derived MPTF in recurrent DVT were significantly increased relative to other MPTF (P<0.05), although there was no difference between initial DVT patients and normal individuals. We demonstrated elevated procoagulant activity of platelet-free plasma in DVT patients relative to normal individuals, and a positive correlation with MPTF.
The elevated MPTF could be a potentially predictor for DVT recurrence. Further studies are needed to validate its sensitivity and specificity.

Download full-text


Available from: Yongbo Huang, Aug 26, 2015
1 Follower
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Venous thromboembolism is an episodic disease with an annual incidence of 2 to 3/1000 per year that is associated with a high morbidity and mortality. Risk factors for venous thromboembolism come in many guises. They fit into an extended version of Virchow's triad and they tilt the hemostatic balance toward clot formation. This can be achieved by decreasing blood flow and lowering oxygen tension, by activating the endothelium, by activating innate or acquired immune responses, by activating blood platelets, or by increasing the number of platelets and red blood cells or modifying the concentrations of pro- and anticoagulant proteins in the blood. In this narrative review we will discuss the known common risk factors within this pathophysiological framework.
    Arteriosclerosis Thrombosis and Vascular Biology 03/2012; 32(3):563-8. DOI:10.1161/ATVBAHA.111.242818 · 5.53 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Pulmonary hypertension (PH) is a fatal disease with no treatment options characterized by elevated pulmonary vascular resistance and secondary right ventricular failure. The etiology of PAH is multiple and its pathogenesis is complex. Although the exact role of cellular microparticles (MPs) remains partially understood, there is increasing evidence to suggest an active role for MPs in PH pathophysiology. Patients with PH exhibited higher circulating levels of MPs compared to controls subjects and in vitro or in vivo generated MPs can induce endothelial dysfunction, interfere with coagulation pathways or modulate inflammatory phenomenon. Whether or not these new conveyors of biological information contribute to the acquisition and/or maintenance to the altered endothelial phenotype is unexplored in PH and requires further study.
    European Respiratory Journal 12/2012; 42(1). DOI:10.1183/09031936.00087212 · 7.13 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The paper is an introduction to the issue of endothelial microparticles, their biology and function. The historical view of microparticle research and actual knowledge about microaprticle formation, structure and molecular composition are presented. The new directions of endothelial microparticle research are discussed with the emphasis of their coagulation aspects.
    Postepy biochemii 01/2013; 59(4):395-404.
Show more