Kumar PA, Srinivas TNR, Madhu S, Sravan R, Singh S, Naqvi SWA et al.. Cecembia lonarensis gen. nov., sp. nov., a novel haloalkalitolerant bacterium of the family ‘Cyclobacteriaceae’, isolated from a haloalkaline lake and emended descriptions of the genera Indibacter, Nitritalea and Belliella. Int J Syst Evol Microbiol 62: 2252-2258

Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 11/2011; 62(Pt 9):2252-8. DOI: 10.1099/ijs.0.038604-0
Source: PubMed


A novel Gram-staining-negative, rod-shaped, non-motile bacterium, designated strain LW9(T), was isolated from a water sample collected from Lonar Lake of Buldhana district, Maharashtra, India. Colonies and broth cultures were reddish orange due to the presence of carotenoid pigments. Strain LW9(T) was positive for catalase, ornithine decarboxylase and lysine decarboxylase activities and negative for gelatinase, oxidase, urease and lipase activities. The predominant fatty acids were iso-C(15 : 0) (31.3 %), iso-C(16 : 0) (9.3 %), anteiso-C(15 : 0) (7.3 %), iso-C(16 : 1) H (6.1 %), summed feature 3 (comprising C(16 : 1)ω7c/C(16 : 1)ω6c; 5.9 %), iso-C(17 : 1)ω9c (5.4 %) and iso-C(17 : 0) 3-OH (5.0 %). Strain LW9(T) contained MK-7 as the major respiratory quinone. The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids and seven unidentified lipids. The DNA G+C content of strain LW9(T) was 40.5 mol%. 16S rRNA gene sequence analysis indicated that the type strains of Indibacter alkaliphilus and Aquiflexum balticum, two members of the family Cyclobacteriaceae (phylum 'Bacteroidetes') were the most closely related strains with sequence similarities of 93.0 and 94.0 %, respectively. Other members of the family Cyclobacteriaceae showed sequence similarities <93.0 %. Based on these phenotypic characteristics and on phylogenetic inference, strain LW9(T) is proposed as the representative of novel species in a new genus, Cecembia lonarensis gen. nov., sp. nov. The type strain of the type species, Cecembia lonarensis, is LW9(T) ( = CCUG 58316(T) = KCTC 22772(T)). Emended descriptions of the genera Indibacter, Nitritalea and Belliella are also proposed.

Download full-text


Available from: Shashi Singh, Nov 19, 2015
  • Source
    • "Alkalinization of the extract did not cause a bathychromatic shift of the peak. Therefore, strains NIO-S3 T and NIO-S4 contained carotenoids pigments but no flexirubin pigments, as often observed for marine members of the phylum Bacteroidetes [3] [33]. The cellular fatty acid composition of "
    [Show abstract] [Hide abstract]
    ABSTRACT: Novel orange-pigmented, Gram-negative, rod-shaped, non-motile bacteria, designated strains NIO-S3(T) and NIO-S4, were isolated from a water sample collected from Cochin back waters, Thanneermukkom and Arookutty, Kerala, India. Both strains were positive for oxidase and catalase activities, and hydrolyzed gelatin and Tween 40. The predominant fatty acids were iso-C(15:0), anteiso-C(15:0), iso-C(17:0) 3OH, C(16:1)ω7c/C(16:1)ω6c (summed feature 3) and iso-C(17:1)ω9c/C(16:0) 10-methyl (summed feature 9), whereas MK-7 was the major respiratory quinone, and phosphatidylethanolamine, two unidentified phospholipids and one unidentified lipid were the only polar lipids. The DNA G+C content of the two strains was 43.7 and 43.6mol%, respectively. The 16S rRNA gene sequence analysis indicated that they were members of the genus Algoriphagus and closely related to Algoriphagus olei CC-Hsuan-617(T), Algoriphagus aquatilis A8-7(T), Algoriphagus aquaeductus LMG 24398(T) and Algoriphagus mannitolivorans DSM 15301(T), with pairwise sequence similarities of 96.8, 96.6, 96.2 and 96.2%, respectively. DNA-DNA hybridization between strains NIO-S3(T) and NIO-S4 showed a relatedness of 89%. Based on data from the current polyphasic study, the strains are proposed as a novel species of the genus Algoriphagus, for which the name Algoriphagus shivajiensis sp. nov. is proposed. The type strain of A. shivajiensis is NIO-S3(T) (=JCM 17885(T)=MTCC 11066(T)).
    Systematic and Applied Microbiology 01/2013; 36(2). DOI:10.1016/j.syapm.2012.11.006 · 3.28 Impact Factor
  • Source
    • "The absorption spectrum of the acetone extract of strain AK6 T showed a broad peak with a maximum at approxi - mately 480 nm , which was typical for carotenoid pigments ( Anil Kumar et al . , 2012 ; Asker et al . , 2007 ) . The cellular fatty acid composition of strain AK6 T was dominated by branched saturated and hydroxy fatty acids , especially iso - C 15 : 0 , iso - C 17 : 1 v9c , iso - C 15 : 1 G , iso - C 17 : 0 3 - OH and anteiso - C 15 : 0 . This profile was quite different from that of Aquiflexum balticum DSM 16537 T ( Ta"
    [Show abstract] [Hide abstract]
    ABSTRACT: A novel marine, Gram-staining-negative, oxidase- and catalase- positive, rod-shaped bacterium, designated strain AK6T, was isolated from marine aquaculture pond water collected in Andhra Pradesh, India. The fatty acids were dominated by iso-C15:0, iso-C17:1ω9c, iso-C15:1 G, iso-C17:0 3OH and anteiso-C15:0. Strain AK6T contained MK-7 as the sole respiratory quinone and phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid and six unidentified lipids as polar lipids. The DNA G + C content of strain AK6T was 45.6 mol%. Phylogenetic analysis showed that strain AK6T formed a distinct branch within the family Cyclobacteriaceae and clustered with Aquiflexum balticum DSM 16537T and other members of the family Cyclobacteriaceae. 16S rRNA gene sequence analysis confirmed that Aquiflexum balticum DSM 16537T was the nearest neighbor, with pairwise sequence similarity of 90.1%, while sequence similarity with the other members of the family was < 88.5%. Based on differentiating phenotypic characteristics and phylogenetic inference, strain AK6T is proposed as a representative of a new genus and species of the family Cyclobacteriaceae as Mariniradius saccharolyticus gen. nov., sp. nov. The type strain is AK6T (= MTCC 11279T = JCM 17389T). Emended descriptions of the genus Aquiflexum and Aquiflexum balticum are also proposed.
    International Journal of Systematic and Evolutionary Microbiology 10/2012; 63(Pt 6). DOI:10.1099/ijs.0.043919-0 · 2.51 Impact Factor
  • Source
    • "Emended description of the genus Cecembia Anil Kumar et al. 2012 Characteristics are as given for the description of the genus by Anil Kumar et al. (2012), with the following amendments. Some species are slightly thermophilic. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Two isolates, designated RQ-33(T) and TU-13, were recovered from hot spring runoffs on the Island of São Miguel in the Azores, Portugal. These organisms have an optimum growth temperature of about 45 °C and an optimum pH for growth between 7.5 and 8.5. Based on 16S rRNA gene sequence analysis the new organism is most closely related to the type strain of Cecembia lonarensis at 96.4% pairwise similarity. Strains are red-pigmented, form non-motile rod-shaped cells that stain Gram-negative; the organisms are strictly aerobic, oxidase and catalase positive. The major fatty acids are iso-C15:0 and C15:0. Phosphatidylethanolamine is the major polar lipid. Menaquinone 7 is the major respiratory quinone. This organism from the Azores represents a new species of the genus Cecembia based on the phylogenetic, physiological and biochemical characteristics for which we propose the name Cecembia calidifontis with strain RQ-33(T) (=DSM 21411(T) =LMG 24596(T)) as the type strain. We also propose to emend the description of the genus Cecembia.
    International Journal of Systematic and Evolutionary Microbiology 07/2012; 63(Pt 4). DOI:10.1099/ijs.0.044537-0 · 2.51 Impact Factor
Show more