Cecembia lonarensis gen. nov., sp. nov., a haloalkalitolerant bacterium of the family Cyclobacteriaceae, isolated from a haloalkaline lake and emended descriptions of the genera Indibacter, Nitritalea and Belliella.
ABSTRACT A novel Gram-staining-negative, rod-shaped, non-motile bacterium, designated strain LW9(T), was isolated from a water sample collected from Lonar Lake of Buldhana district, Maharashtra, India. Colonies and broth cultures were reddish orange due to the presence of carotenoid pigments. Strain LW9(T) was positive for catalase, ornithine decarboxylase and lysine decarboxylase activities and negative for gelatinase, oxidase, urease and lipase activities. The predominant fatty acids were iso-C(15 : 0) (31.3 %), iso-C(16 : 0) (9.3 %), anteiso-C(15 : 0) (7.3 %), iso-C(16 : 1) H (6.1 %), summed feature 3 (comprising C(16 : 1)ω7c/C(16 : 1)ω6c; 5.9 %), iso-C(17 : 1)ω9c (5.4 %) and iso-C(17 : 0) 3-OH (5.0 %). Strain LW9(T) contained MK-7 as the major respiratory quinone. The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids and seven unidentified lipids. The DNA G+C content of strain LW9(T) was 40.5 mol%. 16S rRNA gene sequence analysis indicated that the type strains of Indibacter alkaliphilus and Aquiflexum balticum, two members of the family Cyclobacteriaceae (phylum 'Bacteroidetes') were the most closely related strains with sequence similarities of 93.0 and 94.0 %, respectively. Other members of the family Cyclobacteriaceae showed sequence similarities <93.0 %. Based on these phenotypic characteristics and on phylogenetic inference, strain LW9(T) is proposed as the representative of novel species in a new genus, Cecembia lonarensis gen. nov., sp. nov. The type strain of the type species, Cecembia lonarensis, is LW9(T) ( = CCUG 58316(T) = KCTC 22772(T)). Emended descriptions of the genera Indibacter, Nitritalea and Belliella are also proposed.
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ABSTRACT: A novel actinomycete was isolated from compost soil and was studied taxonomically and phylogenetically. Cells of this organism were gram positive, not acid fast, nonmotile, nonsporulating, irregular coccoid to short rod shaped, and microaerophilic. The cell wall peptidoglycan contained lysine and was cross-linked via an L-Lys<--L-Ser<--D-Asp interpeptide bridge. The major menaquinone was MK-8(H4). The polar lipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and two unknown phospholipids. Mycolic acids were absent. The cellular fatty acid profile was complex, with large amounts of saturated and monounsaturated straight-chain acids and smaller amounts of iso and anteiso branched-chain acids. The G+C content of the DNA was 66 mol%. Comparative 16S ribosomal DNA studies revealed that strain HKI 0089T represents a novel lineage within Actinobacteria (32) distinct from all previously described genera and most closely related to members of the genera Kytococcus, Dermacoccus, and Dermatophilus of the family Dermatophilaceae. On the basis of our results, we suggest that strain HKI 0089 should be classified in a new genus and species, for which we propose the name Demetria terragena. The type strain and the only strain of the genus and species is HKI 0089 (DSM 11295).International journal of systematic bacteriology 10/1997; 47(4):1129-33. · 2.27 Impact Factor
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ABSTRACT: The marine bacterial strain HY9(T) was isolated from sediment from the South China Sea. Strain HY9(T) is aerobic, heterotrophic and rose-pigmented. The cells are non-motile and curved, i.e. ring-like or horseshoe-shaped. The 16S rRNA gene sequence of strain HY9(T) was determined and blast searches revealed that it possessed significant sequence similarities with respect to Cyclobacterium species (92.8-93.6 %). Phylogenetic analysis confirmed that strain HY9(T) was tightly clustered with members of the genus Cyclobacterium. The cellular morphology and chemotaxonomic and phenotypic properties of strain HY9(T) showed that it should be classified as a member of the genus Cyclobacterium. Significant evolutionary distances and a range of phenotypic features distinguished strain HY9(T) from previously described Cyclobacterium species. Hence, strain HY9(T) represents a novel species in the genus Cyclobacterium, for which the name Cyclobacterium lianum sp. nov. is proposed. The type strain is HY9(T) (=CGMCC 1.6102(T)=JCM 14011(T)). On the basis of this study and previously described properties of Cyclobacterium species, an emended description of the genus Cyclobacterium is proposed.International journal of systematic and evolutionary microbiology 01/2007; 56(Pt 12):2927-30. · 2.11 Impact Factor
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ABSTRACT: We obtained carotenoid-producing microorganisms at a high frequency from water samples collected at Misasa (Tottori, Japan), a region known for its high natural radioactivity content. A comprehensive 16S rRNA gene-based phylogenetic analysis revealed that the 104 potential carotenoid producers isolated from Misasa could be classified into 38 different species belonging to seven bacterial classes (Flavobacteria, Sphingobacteria, alpha-Proteobacteria, gamma-Proteobacteria, Deinococci, Actinobacteria, and Bacilli). Of these 38 species, 14 showed sequence similarities less than 97% to their closest identified relatives, and 9 were related to genera that have not been described earlier in terms of carotenoid production. The red-pigmented isolates belonging to Deinococci showed marked resistance to gamma rays and UV irradiation, while those related to Sphingomonas showed weak resistance. The carotenoids produced by the isolates were zeaxanthin (6 strains), dihydroxyastaxanthin (24 strains), astaxanthin (27 strains), canthaxanthin (10 strains), and unidentified molecular species that were produced by the isolates related to Deinococcus, Exiguobacterium, and Flectobacillus. UV irradiation would be useful for the selective isolation of carotenoid-producing microorganisms, and that new microbial producers and other molecular species of carotenoids may potentially be identified from radioactive environments.Applied Microbiology and Biotechnology 12/2007; 77(2):383-92. · 3.69 Impact Factor