José, A, Sobrevals, L, Ivorra, A and Fillat, C. Irreversible electroporation shows efficacy against pancreatic carcinoma without systemic toxicity in mouse models. Cancer Lett 317: 16-23

Institut d'Investigacions Biomèdiques August Pi i Sunyer-IDIBAPS, Barcelona, Spain.
Cancer letters (Impact Factor: 5.62). 11/2011; 317(1):16-23. DOI: 10.1016/j.canlet.2011.11.004
Source: PubMed


Pancreatic ductal adenocarcinoma (PDAC) therapies show limited success. Irreversible electroporation (IRE) is an innovative loco-regional therapy in which high-voltage pulses are applied to induce plasma membrane defects leading to cellular death. In the present study we evaluated the feasibility of IRE against PDAC. IRE treatment exhibited significant antitumor effects and prolonged survival in mice with orthotopic xenografts. Extensive tumor necrosis, reduced tumor cell proliferation and disruption of microvessels were observed at different days post-IRE. Animals had transient increases in transaminases, amylase and lipase enzymes that normalized at 24h post-IRE. These results suggest that IRE could be an effective treatment for locally advanced pancreatic tumors.

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    • "Male athymic nu/nu mice (8 weeks old, Harlan Iberica) were used to generate orthotopic pancreatic tumors. PANC-1 or PANC- 1/CAF-25, and PANC-1-Luc/CAF-28 mixed orthotopic tumors were generated by injecting a total of 350,000 cells (ratio 10:1) into the pancreas of athymic nude mice, in a final volume of 50 μl, as previously described [17] "
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    • "Four days post NTIRE, lower emission was monitored in the treated animals than in the control. Moreover , at the end of the experiment complete tumor eradication was achieved in 25% of the mice [53]. In the second work, green-florescent-protein (GFP) was injected in to pig liver before NTIRE. "
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