Article

Identification of a novel single-stranded, circular DNA virus from bovine stool.

Department of Veterinary Virology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
Journal of General Virology (impact factor: 3.36). 11/2011; 93(Pt 3):635-9. DOI:10.1099/vir.0.037838-0 pp.635-9
Source: PubMed

ABSTRACT We report the identification of a novel single-stranded, circular DNA virus isolated from bovine stool. The virus, named bovine stool-associated circular DNA virus (BoSCV), has a genome comprising 2600 bases of circular ssDNA, with two putative ORFs encoding replicase and capsid proteins, arranged inversely. The stem-loop structure was located between the 3' ends of the two putative ORFs, as in chimpanzee stool-associated circular virus (ChimpSCV) and unlike other circular DNA viruses, including members of the families Circoviridae, Nanoviridae and Geminiviridae. BoSCV was also genetically similar to ChimpSCV, with approximately 30 % identity in the replicase and capsid proteins. A phylogenetic analysis based on the replicase protein showed that BoSCV and ChimpSCV are in the same clade. A field survey using BoSCV-specific PCRs targeting ORF1 detected BoSCV and BoSCV-like sequences in bovine and porcine stool samples. BoSCV appears to belong to a new genus of circular DNA viruses.

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Keywords

BoSCV
 
BoSCV-like sequences
 
BoSCV-specific PCRs
 
bovine
 
bovine stool
 
bovine stool-associated circular DNA virus
 
capsid proteins
 
chimpanzee stool-associated circular virus
 
circular DNA virus
 
circular DNA viruses
 
circular ssDNA
 
families Circoviridae
 
genome
 
new genus
 
phylogenetic analysis
 
porcine stool samples
 
putative ORFs encoding replicase
 
replicase protein
 
stem-loop structure
 
two putative ORFs
 

Hye Kwon Kim