Article

Probing proteomes with benzophenone photoprobes.

Department of Chemistry, Hunter College of CUNY, New York, NY, USA.
Methods in molecular biology (Clifton, N.J.) 01/2012; 803:65-75. DOI:10.1007/978-1-61779-364-6_6
Source: PubMed

ABSTRACT Benzophenone photoprobes represent powerful tools for chemical proteomics. Upon UV irradiation, a benzophenone photoprobe can selectively form a covalent bond with its target protein in complex protein mixtures. Thus, photoprobes can be used to profile a wide variety of proteins in complex proteomes. This chapter describes simple protocols to derivatize fluorenylmethyloxycarbonyl (Fmoc)-protected peptide-nucleic-acid adenine (PNA adenine) into a benzophenone photoprobe and its application in photolabeling its target proteins. The method as described does not require specialized equipment for probe synthesis and photolabeling. In addition, the strategy is applicable to recognition motifs other than PNA adenine, such as peptides, to profile their target proteins in complex proteomes.

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Keywords

complex protein mixtures
 
complex proteomes
 
covalent bond
 
Fmoc)-protected peptide-nucleic-acid adenine
 
peptides
 
PNA adenine
 
powerful tools
 
probe synthesis
 
proteins
 
simple protocols
 
specialized equipment
 
target protein
 
target proteins
 
UV irradiation
 
wide variety
 

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