Novel broad-host-range vehicles for cloning and shuffling of gene cassettes

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Pawinskiego 5A, Poland.
Journal of microbiological methods (Impact Factor: 2.1). 10/2011; 88(1):53-62. DOI: 10.1016/j.mimet.2011.10.011
Source: PubMed

ABSTRACT Novel vectors for cloning and shuffling of gene cassettes based on minireplicon of broad-host-range RA3 plasmid from IncU incompatibility group were constructed. A series of minireplicon variants were prepared with copy number ranging from low (1-2 copies per chromosome), medium (10-15 copies per chromosome) to high copy number (80-90 copies per chromosome). The new cloning vectors are relatively small in size (4.5-5.4kb) and carry various resistance determinants: kanamycin (Km(R)), tetracycline (Tc(R)) or chloramphenicol (Cm(R)). The vectors were engineered to facilitate cloning and shuffling of the functional modules with or without transcriptional terminators. Using the described strategy, a bank of functional modules, ready for exchange, has been initiated.

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Available from: Aneta Agnieszka Bartosik, Jun 02, 2014
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