Calorie restriction-like effects of 30 days of resveratrol supplementation on energy metabolism and metabolic profile in obese humans.

Top Institute Food and Nutrition (TIFN), Wageningen, The Netherlands.
Cell metabolism (Impact Factor: 17.35). 11/2011; 14(5):612-22. DOI: 10.1016/j.cmet.2011.10.002
Source: PubMed

ABSTRACT Resveratrol is a natural compound that affects energy metabolism and mitochondrial function and serves as a calorie restriction mimetic, at least in animal models of obesity. Here, we treated 11 healthy, obese men with placebo and 150 mg/day resveratrol (resVida) in a randomized double-blind crossover study for 30 days. Resveratrol significantly reduced sleeping and resting metabolic rate. In muscle, resveratrol activated AMPK, increased SIRT1 and PGC-1α protein levels, increased citrate synthase activity without change in mitochondrial content, and improved muscle mitochondrial respiration on a fatty acid-derived substrate. Furthermore, resveratrol elevated intramyocellular lipid levels and decreased intrahepatic lipid content, circulating glucose, triglycerides, alanine-aminotransferase, and inflammation markers. Systolic blood pressure dropped and HOMA index improved after resveratrol. In the postprandial state, adipose tissue lipolysis and plasma fatty acid and glycerol decreased. In conclusion, we demonstrate that 30 days of resveratrol supplementation induces metabolic changes in obese humans, mimicking the effects of calorie restriction.

1 Bookmark
  • [Show abstract] [Hide abstract]
    ABSTRACT: Acute UVB exposure triggers inflammation leading to the induction of indoleamine 2,3 dioxygenase (IDO1), one of the first enzymes in the kynurenine pathway (KP) for tryptophan degradation. However, limited studies have been undertaken to determine the catabolism of tryptophan within the skin. The aim of this study was two-fold: (1) to establish if the administration of the proinflammatory cytokine interferon-gamma (IFN-γ) and/or UVB radiation elicits differential KP expression patterns in human fibroblast and keratinocytes, and (2) to evaluate the effect of KP metabolites on intracellular nicotinamide adenine dinucleotide (NAD+) levels, and cell viability. Primary cultures of human fibroblasts and keratinocytes were used to examine expression of the KP at the mRNA level using qPCR, and at the protein level using immunocytochemistry. Cellular responses to KP metabolites were assessed by examining extracellular lactate dehydrogenase (LDH) activity and intracellular NAD+ levels. Major downstream KP metabolites were analysed using GC/MS and HPLC. Our data shows that the KP is fully expressed both in human fibroblasts and keratinocytes. Exposure to UVB radiation and/or IFN-γ causes significant changes in the expression pattern of downstream KP metabolites and enzymes. Exposure to various concentrations of KP metabolites showed marked differences in cell viability and intracellular NAD+ production, providing support for involvement of the KP in the de novo synthesis of NAD+ in the skin. This new information will have a significant impact on our understanding of the pathogenesis of UV related skin damage and the diagnosis of KP related disease states. This article is protected by copyright. All rights reserved
    Journal of Cellular Biochemistry 01/2015; DOI:10.1002/jcb.25019 · 3.37 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Respiratory chambers are the current gold standard for assessing human energy expenditure and substrate utilization over a long period of time (several hours to several days), based on oxygen consumption, carbon dioxide production, and urinary nitrogen excretion. Analysis of human energy metabolism using a respiratory chamber provides information about the total energy expenditure (TEE), sleeping metabolic rate (SMR), resting metabolic rate, diet-induced thermogenesis (DIT), activity-induced thermogenesis (AIT), and substrate oxidation. In this review, we describe the theoretical underpinnings of the respiratory chamber, as well as the measurement reproducibility and applications as study endpoints for indirect calorimetry. In humans, the coefficients of variation in energy expenditure and substrate utilization were estimated by 24-h repeatability studies. Under the appropriate conditions, the coefficients of variation for TEE were 1% to 5%, SMR was around 1%, DIT was around 40%, AIT was around 10%, and substrate oxidation was around 5%. Factors that impact energy expenditure and sub-strate oxidation have been reported, and future weight changes can be predicted based on the 24-h respiratory quotient and substrate oxidation. As the 24-h energy expenditure and substrate oxidation are affected by the 24-h energy balance, it is important to consider the subject's energy balance prior to and during calorimetry. Accurate measurements of energy and substrate balance (intake minus utilization) will contribute to a better understanding of the conditions that lead to changes in body weight. Properly obtaining measurements using a respiratory chamber requires a thorough understanding of the measurement principles and calculation methods, as well as an appropriate protocol. Keywords : indirect calorimetry, coefficient of variance, energy balance, energy expenditure components , substrate oxidation
    01/2013; 2(1):93-99. DOI:10.7600/jpfsm.2.93
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Pre-clinical findings have provided mounting evidence that resveratrol, a dietary polyphenol, may confer health benefits and protect against a variety of medical conditions and age-related complications. However, there is no consistent evidence of an increased protection against metabolic disorders and other ailments when comparing studies in laboratory animals and humans. A number of extraneous and potential confounding variables can affect the outcome of clinical research. To date, most of the studies that have investigated the effect of resveratrol administration on patient outcomes have been limited by their sample sizes. In this review we will survey the latest advances regarding the timing, dosage, formulation, bioavailability, and toxicity of resveratrol, and resveratrol-drug interactions in human studies. Moreover, the present report focuses on the actions of resveratrol treatment in combatting diseases, such as cancer, diabetes, neurodegeneration, cardiovascular disease, and other age-related ailments.
    Ageing research reviews 01/2015; 21. DOI:10.1016/j.arr.2015.01.002 · 7.63 Impact Factor

Full-text (2 Sources)

Available from
May 31, 2014